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ST1553

Sigma-Aldrich

Anti-VCP Mouse mAb (4A8)

liquid, clone 4A8, Calbiochem®

Synonym(s):
Anti-Valosin-Containing Protein

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4A8, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... VCP(7415)

General description

Recognizes the ~67 kDa VCP protein in HePG2 cells and human placenta tissue.
Purified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.
This Anti-VCP Mouse mAb (4A8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of VCP.

Immunogen

Full-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein

Application



ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HePG2 cells, human placenta tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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