ST1643
Anti-PDK2 Mouse mAb (2G1)
liquid, clone 2G1, Calbiochem®
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Synonym(s):
Anti-Pyruvate Dehydrogenase Kinase 2
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2G1, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PDK2(5164)
General description
Purified mouse monoclonal antibody. Recognizes the ~40 kDa PDK2 protein.
Recognizes the ~40 kDa PDK2 protein in U-2 OS cells.
This Anti-PDK2 Mouse mAb (2G1) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PDK2.
Immunogen
A recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.8 g/ml)
Warning
Toxicity: Regulatory Review (Z)
Physical form
In PBS, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
U-2 OS cells
U-2 OS cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Jakob G Knudsen et al.
Physiological reports, 4(9) (2016-05-18)
Interleukin (IL)-6 is released from skeletal muscle (SkM) during exercise and has been shown to affect hepatic metabolism. It is, however, unknown whether SkM IL-6 is involved in the regulation of exercise training-induced counteraction of changes in carbohydrate and lipid
Anders Gudiksen et al.
PloS one, 11(6), e0156460-e0156460 (2016-06-22)
Pyruvate dehydrogenase (PDH) plays a key role in the regulation of skeletal muscle substrate utilization. IL-6 is produced in skeletal muscle during exercise in a duration dependent manner and has been reported to increase whole body fatty acid oxidation, muscle
Anders Gudiksen et al.
Physiological reports, 5(7) (2017-04-13)
The purpose of the present study was to examine whether lack of skeletal muscle peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) affects the switch in substrate utilization from a fed to fasted state and the fasting-induced pyruvate dehydrogenase (PDH)
Anders Gudiksen et al.
American journal of physiology. Endocrinology and metabolism, 312(3), E204-E214 (2016-12-29)
Fasting prompts a metabolic shift in substrate utilization from carbohydrate to predominant fat oxidation in skeletal muscle, and pyruvate dehydrogenase (PDH) is seen as a controlling link between the competitive oxidation of carbohydrate and fat during metabolic challenges like fasting.
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