ST1643

Anti-PDK2 Mouse mAb (2G1)

liquid, clone 2G1, Calbiochem^®

• Synonym(s): Anti-Pyruvate Dehydrogenase Kinase 2
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 2G1, monoclonal
• Species reactivity: human
• Application: —
• Citations: 4

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2G1, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (ELISA ; Immunoblotting (1-5 µg/mL); Paraffin Sections (0.8 µg/mL))
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PDK2(5164)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~40 kDa PDK2 protein.

Recognizes the ~40 kDa PDK2 protein in U-2 OS cells.

This Anti-PDK2 Mouse mAb (2G1) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PDK2.

Immunogen

A recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.8 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
U-2 OS cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable