MilliporeSigma
All Photos(2)

Documents

TGFBMAG-64K-01

Millipore

MILLIPLEX® TGFß1 Magnetic Bead Single Plex Kit - Immunology Multiplex Assay

This Immunology Multiplex Assay is used for the simultaneous quantification of the TGF-β1 biomarker in the following species: Mouse/Human/Rat/Pig/Horse/Rabbit/Guinea Pig/Hamster.

All Photos(2)
Synonym(s):
Luminex® cytokine immunoassay, Millipore TGF beta kit, transforming growth factor beta 1 kit
eCl@ss:
32161000

Quality Level

200

species reactivity

rat, canine, feline, nonhuman primates, mouse, human, pig

manufacturer/tradename

Milliplex®

assay range

accuracy: 100%
sensitivity: 12 pg/mL
standard curve range: 9.8-2,500 pg/mL

technique(s)

multiplexing: suitable

compatibility

configured for Single Plex

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
48-614MAGMCYTMAG-70K-PX32HCYTOMAG-60K
MILLIPLEX® TGFß1 Magnetic Bead Single Plex Kit - Immunology Multiplex Assay This Immunology Multiplex Assay is used for the simultaneous quantification of the TGF-β1 biomarker in the following species: Mouse/Human/Rat/Pig/Horse/Rabbit/Guinea Pig/Hamster.

Millipore

TGFBMAG-64K-01

MILLIPLEX® TGFß1 Magnetic Bead Single Plex Kit - Immunology Multiplex Assay

Millipore

Millipore

48-614MAG

MILLIPLEX® TGFβ Signaling Pathway Magnetic Bead 6-Plex - Cell Signaling Multiplex Assay

MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

Millipore

MCYTMAG-70K-PX32

MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay

MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

Millipore

HCYTOMAG-60K

MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 100%, sensitivity: 12 pg/mL, standard curve range: 9.8-2,500 pg/mL

assay range

-

assay range

accuracy: 85-107%, standard curve range: 3.2-10,000 pg/mL

assay range

accuracy: 87-107%, standard curve range: 3.2-10,000 pg/mL
(sCD40L, EGF, Eotaxin/CCL11, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1, MCP-3, MDC (CCL22), MIP-1α, MIP-1β, PDGF-AB/BB, RANTES, TGF-α, TNF-α, TNF-β, VEGF), inter-assay cv: 5-19%
intra-assay cv: 2-13%

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

compatibility

configured for Single Plex

compatibility

-

compatibility

configured for Premixed

compatibility

-

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex® xMAP®)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

General description

The transforming growth factor beta (TGFβ) system, a superfamily of cytokines as well as signaling pathways, is highly conserved throughout the animal kingdom. TGFβ functions in immune cell development, angiogenesis, wound healing and embryonic development, bone development and plays a critical role in immune response, heart disease, and cancer. In its normal state TGFβ is one of the few classes of proteins able to inhibit cell growth by halting mitosis at the G1 state, inducing cell differentiation or apoptosis. However, during oncogenesis mutations in the TGFβ signaling pathway result in tumor cell resistance to the effects of normally functioning TGFβ, causing proliferation without regulation. Initial research suggests that VE-cadherin may enhance the mutated TGFβ signaling pathway.

The MILLIPLEX® TGFβ1 Single Plex Magnetic Bead Kit is a single plex kit to be used for the quantification of TGFβ in serum, plasma, tissue/cell lysate and culture supernatant
samples.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

In addition to human TGFβ1, the assay can detect TGFβ1 in serum of the following; horse, rabbit, mouse, rat, mini pig, canine, hamster, guinea pig, rhesus monkey and cynomolgous monkey. However, the exact amount of cross reactivity has not been determined.

Application

  • Analyte: TGFβ1
  • Recommended Species: In addition to human TGFβ1, the assay can detect TGFβ1 in serum of the following: horse, rabbit, mouse, rat, mini pig, canine, hamster, guinea pig, rhesus monkey and cynomolgus monkey. However, the exact amount of cross reactivity has not been determined.
  • Recommended Sample Type: Serum, plasma, cell/tissue culture supernatants and lysates
  • NOTE: This assay requires a sample acidification treatment using the provided reagents and protocol.
  • Recommended Sample Dilution: 25 μL per well of a 1:30 dilution after acidification treatment of serum and plasma samples; cell/tissue culture samples may require dilution prior to acidification treatment (see protocol for details).
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or alternatively, 2 hours at room temperature (20-25°C).
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Cardiovascular Disease, Chronic Inflammatory Disease, Immune Disorders, Inflammatory Disease, Rheumatoid Arthritis

Packaging

Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - Skin Sens. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Nancy N Ortiz-Villatoro et al.
Scientific reports, 11(1), 16780-16780 (2021-08-20)
There are no clinical interventions to prevent post-injury epilepsy, a common and devastating outcome after brain insults. Epileptogenic events that run from brain injury to epilepsy are poorly understood. Previous studies in our laboratory suggested Proechimys, an exotic Amazonian rodent
Ling Tong et al.
Frontiers in microbiology, 12, 704449-704449 (2021-08-06)
It is challenging to trace the complicated individual-based variations of HIV-specific immunocompetence shift during the successful antiretroviral therapy (ART) era. Using eight rhesus monkeys simulating a longitudinal stage-dependent cohort (baseline-SIV acute infection-SIV suppression by ART-ART withdrawal), baseline immunocompetence monitoring for
Patrick Bellelis et al.
Gynecologic and obstetric investigation, 84(5), 435-444 (2019-02-04)
The objective of this study was to evaluate cytokines related to natural killer and T-regulatory cells in endometriotic lesions, peritoneal fluid (PF) and the peripheral blood (PB) of patients with deep infiltrative endometriosis. A case-control study was conducted in a
Xiaonan Li et al.
PloS one, 16(5), e0251293-e0251293 (2021-05-14)
Proteins and lipids of milk fat globule membrane (MFGM) and probiotics are immunomodulatory. We hypothesized that Lactobacillus paracasei ssp. paracasei strain F19 (F19) would augment vaccine antibody and T helper 1 type immune responses whereas MFGM would produce an immune
Albert D Donnenberg et al.
Oncotarget, 10(60), 6456-6465 (2019-11-20)
We compared the secretome of metastatic (non-small cell lung cancer (NSCLC)) and primary (mesothelioma) malignant pleural effusions, benign effusions and the published plasma profile of patients receiving chimeric antigen receptor T cells (CAR-T), to determine factors unique to neoplasia in
View All Related Papers

Related Content

Multiplexing Cancer & Metastasis Signaling Pathways | MILLIPLEX® Assays

Learn how multiplexing with MILLIPLEX® multiplex assays can help simplify cancer research by measuring multiple circulating biomarkers at once. Find multiplex panels to analyze JAK-STAT, mTOR, MAPK, SAPK, TGFβ signaling pathways, and more.

Multiplexing Inflammation Signaling Pathways | MILLIPLEX® Assays

See how multiplexing the inflammation signaling pathway with MILLIPLEX® inflammation assays or cell signaling assays can help researchers bridge the gap between immunology and cell signaling, including investigating T cell signaling, Th Cell differentiation, inflammatory response signaling, and sepsis signaling.

Multiplex Assays for COVID-19 (SARS-CoV-2) Research Applications | MILLIPLEX® Assays

See how MILLIPLEX® multiplex immunoassays offer researchers the ability to simultaneously quantitate a large number of human cytokines, chemokines, and growth factors, and detect human immunoglobulins against SARS-CoV-2 antigens, to better understand the immune response to COVID-19, including cytokine storm.

Multiplexing in Vet Med and Animal Health Research | MILLIPLEX® Assays

Learn the benefits of using biomarker detection multiplex immunoassays for laboratory, companion, and agricultural animal research and explore MILLIPLEX® multiplex assays for veterinary medicine and animal health research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service