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UFC30DV0S

Millipore

Ultrafree®-MC Centrifugal Filter

0.65 µm pore size, hydrophilic PVDF, 0.5 mL volume, sterile

Synonym(s):
Centrifuge filter
eCl@ss:
36100101
NACRES:
NB.24

material

PVDF membrane (hydrophilic)
polypropylene

Quality Level

sterility

sterile

feature

holdup volume 5 μL

manufacturer/tradename

Ultrafree®

parameter

0.5 mL sample volume
50 °C max. temp.

technique(s)

protein extraction: suitable
protein purification: suitable

L

45 mm

diam.

10.6 mm

filtration area

0.2 cm2

matrix

Durapore®

pore size

0.65 μm pore size

application(s)

sample preparation

shipped in

ambient

General description

Filter Type: Microfiltration
Filter Code: DVPP
Ultrafree®-MC is a single-use, disposable filter used for removing particulates from aqueous biological solutions.

Application

Ultrafree®-MC Centrifugal Filter has been used:
  • to filter extracellular vesicle (EV) from seminal plasma
  • in the purification of single-strand-selective uracil-DNA glycosylase (SMUG1)
  • to the purification of EVs for nanoparticle tracking analysis

Physical form

Color Code: Purple

Legal Information

Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
Ultrafree is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificate of Analysis

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Certificate of Quality

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Ruth E Thomas et al.
PLoS genetics, 14(9), e1007694-e1007694 (2018-09-27)
Mutations in the glucosylceramidase beta (GBA) gene are strongly associated with neurodegenerative diseases marked by protein aggregation. GBA encodes the lysosomal enzyme glucocerebrosidase, which breaks down glucosylceramide. A common explanation for the link between GBA mutations and protein aggregation is
Janis A Müller et al.
Nature communications, 9(1), 2207-2207 (2018-06-09)
Zika virus (ZIKV) causes severe birth defects and can be transmitted via sexual intercourse. Semen from ZIKV-infected individuals contains high viral loads and may therefore serve as an important vector for virus transmission. Here we analyze the effect of semen
Penelope Kroustallaki et al.
Cell reports, 28(7), 1690-1702 (2019-08-15)
Telomerase biogenesis is a complex process where several steps remain poorly understood. Single-strand-selective uracil-DNA glycosylase (SMUG1) associates with the DKC1-containing H/ACA ribonucleoprotein complex, which is essential for telomerase biogenesis. Herein, we show that SMUG1 interacts with the telomeric RNA component

Protocols

Affinity Purification with Ultrafree®-MC Centrifugal Filters

Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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