There is no upper limit for protein load in the Amicons, but researchers must be aware of the solubility of their target protein and empirically determine optimal running conditions.
UFC703008
Centricon® Plus Centrifugal Filter
MWCO 30 kDa, sample volume 15-70 mL, regenerated cellulose membrane
Synonym(s):
Centrifuge concentrator
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| Size/SKU | Availability | Price |
|---|---|---|
8 filters | Check Cart for Availability | $344.00 |
About This Item
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polypropylene cap, polypropylene cup (for concentrate/retentate), polypropylene cup (for filtrate collection), polypropylene cup (for sample filter), regenerated cellulose membrane
Pore size:
30 kDa MWCO
Working volume:
≤70 mL , 15—70 mL
Sterility:
non-sterile
Packaging:
pkg of 8 filter
Quality Segment
material
polypropylene cap, polypropylene cup (for concentrate/retentate), polypropylene cup (for filtrate collection), polypropylene cup (for sample filter), regenerated cellulose membrane
sterility
non-sterile
product line
Amicon®
feature
holdup volume 75 μL
packaging
pkg of 8 filter
manufacturer/tradename
Amicon® Ultra, Centricon®
parameter
15-70 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
121 mm
diam.
6 cm
filtration area
19 cm2
working volume
≤70 mL , 15—70 mL
pore size
30 kDa MWCO
application(s)
sample preparation
shipped in
ambient
General description
Filter Type: Ultrafiltration
The Centricon Plus-70 centrifugal filter is a disposable, single-use device designed for rapid processing of aqueous biological solutions in volumes ranging from 15 to 70 mL. It is compatible with swinging-bucket centrifuges only. The Centricon Plus-70 device can concentrate most 70 mL solutions down to 350 μL in just 25 minutes (some solutions may take slightly longer).
•90% typical recovery
•50X to 200X concentration
•Low hold-up volume
•Polypropylene housing minimizes binding
•Deadstop prevents spinning to dryness
•Ready to use. Nothing to assemble
Applications:
•Concentrating and desalting chromatography column eluates
•Concentrating monoclonal antibodies
•Concentrating proteins or viruses from culture supernatants
•Clarifying tissue homogenates and cell lysates
•Buffer exchange or diafiltration
•90% typical recovery
•50X to 200X concentration
•Low hold-up volume
•Polypropylene housing minimizes binding
•Deadstop prevents spinning to dryness
•Ready to use. Nothing to assemble
Applications:
•Concentrating and desalting chromatography column eluates
•Concentrating monoclonal antibodies
•Concentrating proteins or viruses from culture supernatants
•Clarifying tissue homogenates and cell lysates
•Buffer exchange or diafiltration
Application
30 kDa cutoff, 15 mL to 70 mL volume
Features and Benefits
Minimum Final Concentrate Volume: 350 µL
Physical form
Color Code: Clear
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
CENTRICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
This Item | |||
|---|---|---|---|
| description MWCO 30 kDa, sample volume 15-70 mL, regenerated cellulose membrane | description MWCO 10 kDa, sample volume 15-70 mL, regenerated cellulose membrane | description MWCO 100 kDa, sample volume 15-70 mL, regenerated cellulose membrane | description NMWCO 10 kDa, Ultracel® regenerated cellulose membrane (low binding), sample volume 0.5 mL |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| parameter 15-70 mL sample volume | parameter 15-70 mL sample volume | parameter 15-70 mL sample volume | parameter 0.5 mL sample volume |
| pore size 30 kDa MWCO | pore size 10 kDa MWCO | pore size 100 kDa MWCO | pore size 10 kDa NMWCO |
| manufacturer/tradename Centricon®, Amicon® Ultra | manufacturer/tradename Amicon® Ultra, Centricon® | manufacturer/tradename Centricon®, Amicon® Ultra | manufacturer/tradename Microcon® |
| material polypropylene cup (for sample filter), polypropylene cap, polypropylene cup (for concentrate/retentate), regenerated cellulose membrane, polypropylene cup (for filtrate collection) | material polypropylene cup (for filtrate collection), polypropylene cap, polypropylene cup (for concentrate/retentate), regenerated cellulose membrane, polypropylene cup (for sample filter) | material polypropylene cup (for concentrate/retentate), polypropylene cap, polypropylene cup (for filtrate collection), regenerated cellulose membrane, polypropylene cup (for sample filter) | material Ultracel® regenerated cellulose membrane (low binding), silicone O-ring (medical-grade), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate) |
| working volume 15—70 mL , ≤70 mL | working volume ≤70 mL , 15—70 mL | working volume ≤70 mL , 15—70 mL | working volume 0.5 mL |
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Storage Class
10-13 - German Storage Class 10 to 13
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What is the maximum recommended milligram amount of antibody per 70mL 30K in order to prevent the protein from crashing out?
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