No data is available on the maximum or minimum temperature for using the Amicon Ultra-15. Results shown in the user guides are based on experiments conducted at approximately 25°C, not with refrigerated centrifuges. Some users work at 4°C, but filtration times are longer. No studies have been performed at 0°C. Glycerol is compatible with this product.
UFC9010
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 10 kDa
Synonym(s):
10 kDa MWCO, 15 mL sample volume, Centrifugal concentrator
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8 PKG
$128.00
24 PKG
$377.00
96 PKG
$1,470.00
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8 PKG
$128.00
24 PKG
$377.00
96 PKG
$1,470.00
About This Item
UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
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material
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
15 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
12.1 cm (4.807 in.)
diam.
2.97 cm
filtration area
7.6 cm2
volume
15 mL
working volume
≤15 mL
15 mL
pore size
10 kDa MWCO
greener alternative category
shipped in
ambient
1 of 4
This Item | UFC5010 | UFC8010 | UFC201024 |
|---|---|---|---|
| parameter 15 mL sample volume | parameter 0.5 mL sample volume | parameter 4 mL sample volume | parameter 2 mL sample volume |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
General description
Amicon® Ultra 15 mL centrifugal devices with Ultracel® 10,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10-15 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipettor sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Physical form
Color Code: Clear with pink cap
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Is there a recommended temperature for the amicon ultra-15? I would like to concentrate protein at 0 degrees C. Also, is glycerol able to pass through the 30kD filter? The enzyme I want to concentrate is in 50% glycerol. Thanks!
1 answer-
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Can I concentrate cell-propagated respiratory syncytial virus stock using the Amicon® Ultra Centrifugal Filter, 10 kDa MWCO? what is your though and suggestion?
1 answer-
Concentrating cell-propagated respiratory syncytial virus stock with this product has not been studied. However, it can be attempted, as other viruses such as SARS, AAV, and Lenti have been successfully concentrated. The product concentrates substances larger than the molecular weight cut-off of the membrane. It is recommended to consult online research articles using keywords like "Amicon and respiratory syncytial virus" on platforms such as PubMed or Google Scholar.
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Do you make a miniature version of the Amicon® Ultra Centrifugal Filter for 1.5ml samples?
1 answer-
Yes, product UFC5010, the Amicon Ultra 2.0 for samples of 0.5 - 2.0 mL, will accommodate a 1.5 mL sample size. Please see the link below to review this product option:
https://www.sigmaaldrich.com/product/mm/ufc5010Helpful?
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Does ultrafiltration of protein samples result in lower migration on an SDS-PAGE gel?
1 answer-
Yes, ultrafiltration of protein samples can potentially lead to lower migration on an SDS-PAGE gel.During the ultrafiltration process, the desalting or buffer exchange step may remove or reduce sodium chloride (NaCl), leading to lower protein migration on the gel. To prepare protein samples for gel electrophoresis, using an Amicon Ultra (AU) device or a suitable desalting/buffer exchange method is recommended.
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Hello, How much filters represent 1pkg? Only one filter? A full pack? Thank you,
1 answer-
Amicon® Ultra-15 Centrifugal Filter Unit comes in 8, 24, and 96/pk. the pack size is indicated in the last two digits of the catalog numbers.
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Hello, I'm using UFC903096 (Amicon-15, 30K) and am trying to exchange and concentrate without over-concentrating <500uL ... Would you have a table of the volume to add to the filtrate collector to ensure ~ final product volumes. Thanks!
1 answer-
This product is suitable for sample volumes of less than 15 mL. Please see the link below to review the estimated volumes:
https://www.sigmaaldrich.com/products/protein-biology/protein-sample-prep/amicon-centrifugals#howtoselectHelpful?
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How to wash the column after one time used for the buffer exchange or for protein concentration. Is any protocol please share??
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We do not recommend reusing the device because eventually the membrane will clog completely. We have yet to record an efficient way to clean the pores of the membrane without damaging the membrane itself.
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