03010040001

PVDF Western Blotting Membranes

roll W × L 30 cm × 3.00 m, roll of 1, pore size 0.2 μm

• Synonym(s): PVDF Blotting Membranes, Polyvinylidene Fluoride Membranes, western blotting membranes, pvdf
• UNSPSC Code: 41105339
• NACRES: NB.22

Properties

• material: PVDF ; white PVDF membrane (hydrophobic)
• Quality Level: 100
• packaging: roll of 1
• manufacturer/tradename: Roche
• roll W × L: 30 cm × 3.00 m
• pore size: 0.2 μm pore size
• capacity: 131 μg/cm² (BSA); 294 μg/cm² (Goat IgG); 85 μg/cm² (Insulin)
• shipped in: ambient
• storage temp.: room temp

Description

General description

PVDF Western Blotting Membranes are hydrophobic, microporous, polyvinylidene difluoride (PVDF) membranes with a pore size of 0.2μm. The PVDF Western Blotting Membranes are ideal for western and dot blots. The high binding capacity, high mechanical strength, and chemical resistance of the PVDF membrane makes it specifically useful for protein blotting. The combination of strong sample retention and low background binding generates excellent signal-to-noise ratios in colorimetric and chemiluminescent detection. The primary binding mechanism includes electrostatic and hydrophobic nature.
Adsorption: Goat IgG: 294μg/cm²; BSA: 131μg/cm²; insulin: 85μg/cm²

Application

• Dot/slot blotting
• Blotting from 2D gels
• Protein sequencing
• HIV detection
• Hybridoma screening
• Cell blotting
• High binding capacity, high mechanical strength, and chemical resistance of the PVDF membrane makes itespecially useful for protein blotting.
• The combination of strong sample retention with low background binding generates excellent signal-to-noise ratioswith chromogenic and radioactive detection techniques.
• The membrane is also ideal for chemiluminescent substrates.

PVDF Western Blotting Membranes has been used in western blot, for transferring proteins for sequencing.[1][2]
Note: Tested for use with Lumi-Light western blotting substrate or Lumi-Light^Plus western blotting substrate.

Analysis Note

Each lot is function tested (dot blot).

Other Notes

For life science research only. Not for use in diagnostic procedures.