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03359123001

Roche

Alkaline Phosphatase recombinant, highly active

EIA Grade, from Pichia Pastoris

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Enzyme Commission number:

Quality Level

Assay

≥95% (HPLC)

form

buffered aqueous solution (ready-to-use, 20 mg/ml)

specific activity

≥7000 units/mg protein (At 37 °C with pNPP and DEA as the substrates)

packaging

pkg of 10 mg (500 μl)

manufacturer/tradename

Roche

concentration

20 mg/mL (ready-to-use)

optimum pH

8.0(Stability)
9.8(Activity)

shipped in

wet ice

storage temp.

2-8°C

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This Item
A7827AB0100RAP-RO
rAPid Alkaline Phosphatase 1 units/μg protein, optimum reaction temp. 37 °C

Roche

RAP-RO

rAPid Alkaline Phosphatase

form

buffered aqueous solution (ready-to-use, 20 mg/ml)

form

buffered aqueous solution

form

solution

form

-

specific activity

≥7000 units/mg protein (At 37 °C with pNPP and DEA as the substrates)

specific activity

-

specific activity

-

specific activity

1 units/μg protein

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

concentration

20 mg/mL (ready-to-use)

concentration

-

concentration

-

concentration

-

optimum pH

8.0(Stability), 9.8(Activity)

optimum pH

-

optimum pH

-

optimum pH

-

General description

Alkaline phosphatase (ALP) is a hydrolase enzyme. It is a highly active form in calf intestinal mucosa. The calf intestinal alkaline phosphatase is a dimeric glycoprotein with a molecular weight of about 140kDa.
The recombinant, highly active Alkaline Phosphatase possesses the same amino acid sequence as the native, bovine-sourced Alkaline Phosphatase. No animal-derived components are used in the production process. This eliminates the risk of Bovine Spongiform Encephalopathy (BSE) and other infections caused by animals.
The recombinant enzyme guarantees superior lot-to-lot consistency and completely eliminates the risk of BSE.

Specificity

Alkaline phosphatase catalyzed the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccarides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as enzyme purity, enzyme concentration in the assay, buffer, pH etc.

Application

Alkaline Phosphatase can be coupled to other proteins via its amino and carbohydrate groups. The enzyme solution can be used directly for conjugation without prior dialysis. For conjugation of AP with IgG, a ration of 2.5:1 is recommended. The application of the conjugated enzyme varies from ELISAs and microarrays to western blot analysis.

Biochem/physiol Actions

Alkaline phosphatase (ALP) is responsible for removing phosphate groups at the 5- and 3- positions from many types of molecules, such as nucleotides, proteins, and alkaloids. It can also modify lipopolysaccharides by dephosphorylation of the lipid A moiety, thereby making it less toxic than the unmodified lipid A.

Specifications

≥7,000 U/mg (+37°C; pNPP; DEA)

Physical form

Solution in 3 M NaCl, pH 7.6, 5 mM magnesium chloride, 0.1 mM zinc chloride, 30 mM triethanolamine.

Preparation Note

Activator: Mg2-, Co2- and Mn2-ions

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available


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Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota.
Bates JM, et al.
Cell host & microbe, 371-82 null
Quantitation of surface coverage of oligonucleotides bound to chip surfaces: a fluorescence-based approach using alkaline phosphatase digestion.
Pal S, et al.
Lab on a chip, 1332-41 null
Affinity-chromatography purification of alkaline phosphatase from calf intestine.
Brenna O, et al.
The Biochemical Journal, 291-6 null
Intestinal alkaline phosphatase. Physical properties and quaternary structure.
Fosset M, et al.
Biochemistry, 1783-8 null
Yuta Kyosei et al.
Diagnostics (Basel, Switzerland), 10(8) (2020-08-23)
Polymerase chain reaction (PCR)-based antigen tests are technically difficult, time-consuming, and expensive, and may produce false negative results requiring follow-up confirmation with computed tomography. The global coronavirus disease 2019 (COVID-19) pandemic has increased the demand for accurate, easy-to-use, rapid, and

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