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04683633001

Roche

Universal ProbeLibrary Set, Human

(The Universal ProbeLibrary will be phased out by the end of 2020 and stock will be available until it is depleted. The Assay Design Center will be available through December 31st, 2020)

Quality Level

manufacturer/tradename

Roche

packaging

set of of 90 UPL probes, 125 μl, 10 μM, each

General description

The Universal ProbeLibrary Set, Human contains probes #1 to #90 of the 165 Universal ProbeLibrary Probes. In combination with the unique ProbeFinder assay design software, these 90 hydrolysis probes provide real-time PCR assays for more than 98% of the 37,000 human gene transcripts included in the NCBI Reference Sequence Database.
The probes are dual-labeled with a reporter fluorophore (FAM) and a dark quencher dye. They are used in standard real-time PCR assays, on standard real-time PCR instrumentation.
Set of 90 short hydrolysis probes for gene expression quantification of human genes by real-time PCR.

Application

Use the Universal ProbeLibrary Set, Human to obtain rapid and flexible quantification of gene expression levels for virtually all human genes included in the NCBI Reference Sequence Database, in real-time PCR assays on the LightCycler® 480 System, the LightCycler® Carousel-Based System, or other commercially available real-time PCR instruments. Perform an assay with a probe selected from the Universal ProbeLibrary Set, Human by following established real-time PCR protocols using the LightCycler® 480 Probes Master or the LightCycler® TaqMan® Master on the LightCycler® Carousel-Based System, or the FastStart TaqMan® Probe Master (or other real-time PCR-compatible reagents) on other real-time PCR instruments.
Easily design gene-specific expression-quantification assays by using the free web-based ProbeFinder software at the Assay Design Center, accessible at www.universalprobelibrary.com.

Features and Benefits

  • Significantly reduce assay design time and time to result.
  • Combine high assay specificity with flexibility and convenience.
  • No special hardware or unique reaction conditions are required.

Components

90 vials, each containing 125 μl, 10 μM of a Universal ProbeLibrary probe, 5′-end labeled with fluorescein (FAM) and 3′-end labeled with a dark quencher dye.

Quality

All probes in the Universal ProbeLibrary Set, Human have passed a real-time PCR performance test using the matching primers and control templates from the Universal ProbeLibrary Control Set. Each probe is analyzed by anion-exchange HPLC and MALDI-MS to ensure purity and quality.

Legal Information

LightCycler is a registered trademark of Roche
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

RIDADR

NONH for all modes of transport

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis
Certificate of Origin

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