04693116001

Roche

cOmplete Protease Inhibitor Cocktail

Tablets provided in EASYpacks

form

tablet

packaging

pkg of 20 tablets

manufacturer/tradename

Roche

solubility

water: soluble

absorption

0.08 at 280 nm

shipped in

wet ice

storage temp.

2-8°C

General description

cOmplete Protease Inhibitor Tablets inhibit a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains. Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.
cOmplete tablets contain EDTA and both irreversible and reversible protease inhibitors. Protease inhibitors present in cOmplete Tablets do not form irreversible complexes with the SH groups of proteins.

Specificity

Inhibits a broad spectrum of serine proteases, cysteine proteases, and metalloproteases, as well as calpains.

Application

cOmplete Protease Inhibitor Cocktail has been used as an component in
  • extraction buffer for protein extraction from Arabidopsis seedling
  • in radioimmunoprecipitation assay (RIPA) buffer for the isolation of protein from lymphocytes
  • the extraction buffer for the lysis of protoplast.

cOmplete is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
cOmplete contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete to all stock buffers and solutions normally protected with protease inhibitors, and not  just during the initial purification steps.
cOmplete contains EDTA (1 tablet yields a  1 mM EDTA solution  in 50 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+, Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography. Alternatively, the product cOmplete, EDTA-free, can be used. These tablets are identical to cOmplete, with the exception that no EDTA or other chelating agent is  present.
cOmplete Tablets inhibit proteolytic activity in large extraction volumes (up to 50 ml). If you are working with smaller volumes (up to 10 ml), we recommend to use cOmplete, Mini. The composition of the cOmplete, Mini Tablet, is identical to the normal cOmplete Tablet.

Features and Benefits

Use cOmplete Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.

  • Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
  • Complete protection: Instantly protect your proteins against a broad range of proteases.
  • Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
  • Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.

Packaging

20 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters.

Components

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Physical form

Tablets in foil blister packs

Preparation Note

Working concentration: 1 tablet per 50ml extraction solution
Working solution: Handling

Carefully push the tablet through the foil packaging using the base of your thumb (not finger nail) to prevent the breakage of tablets.

Preparation of Working Solution

One cOmplete tablet is sufficient for the inhibition of the proteolytic activity in 50ml extraction solution. When a very high proteolytic activity is present, one tablet should be used for 25ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (25 X conc.) can be prepared.

Preparation of Stock solution (25 X conc.)

Dissolve one cOmplete tablet in 2ml dist. water or in 2ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8°C for 1 to 2 weeks, or at least 12 weeks at -15 to -25°C.

Reconstitution

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

Storage and Stability

Store at 2 to 8 °C. (storage at -15 to -25 °C is also possible)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

cOmplete is a trademark of Roche

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis
Certificate of Origin
ProBDNF/p75NTR/sortilin pathway is activated in peripheral blood of patients with alcohol dependence
Zhou L, et al.
Translational Psychiatry, 7(11), 2-2 (2018)
The receptor-like cytoplasmic kinase BIK1 localizes to the nucleus and regulates defense hormone expression during plant innate immunity
Lal NK, et al.
Cell host & microbe, 23(4), 485-497 (2018)
EL1-like casein kinases suppress ABA signaling and responses by phosphorylating and destabilizing the ABA receptors PYR/PYLs in Arabidopsis
Chen HH, et al.
Molecular plant, 11(5), 706-719 (2018)
Mingwei Ni et al.
Glia, 59(5), 810-820 (2011-02-26)
As the two major glial cell types in the brain, astrocytes and microglia play pivotal but different roles in maintaining optimal brain function. Although both cell types have been implicated as major targets of methylmercury (MeHg), their sensitivities and adaptive...
Hajime Shiba et al.
The Journal of experimental biology, 219(Pt 8), 1146-1153 (2016-03-06)
To elucidate the degradation process of the posterior silk gland during metamorphosis of the silkworm ITALIC! Bombyx mori, tissues collected on the 6th day after entering the 5th instar (V6), prior to spinning (PS), during spinning (SP) and after cocoon...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.