04693124001
Roche
cOmplete™, Mini Protease Inhibitor Cocktail
Tablets provided in EASYpacks
Recommended Products
form
tablet
packaging
pack of 30 tablets (individually packed in foil blister packs)
manufacturer/tradename
Roche
solubility
aqueous buffer: soluble
water: soluble
storage temp.
2-8°C
Related Categories
General description
cOmplete, Mini, contains EDTA and both irreversible and reversible protease inhibitors.
Specificity
Application
- mammalian-protein extraction reagent (M-PER) for lysis of glioma stem cells (GSCs)
- sodium dodecyl sulphate-tris buffered saline (SDS-TBS) for the homogenisation of brain tissues
- radioimmunoprecipitation assay (RIPA) buffer for lysis of human embryonic stem cells (hESCs)
cOmplete, Mini, contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete, Mini, to all stock buffers and solutions normally protected with protease inhibitors, and not just during the initial purification steps.
cOmplete, Mini, contains EDTA (1 tablet yields a 1 mM EDTA solution in 10 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+, Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography.
Alternatively, the product cOmplete, Mini, EDTA-free, can be used. These tablets are identical to cOmplete, Mini, with the exception that no EDTA or other chelating agent is present.
cOmplete, Mini Tablets, inhibit proteolytic activity in small extraction volumes (up to 10 ml). If you are working with a larger volume (up to 50 ml), we recommend to use a different cOmplete product. The composition of the cOmplete Tablet is identical to the cOmplete, Mini Tablet, therefore, comparable results are achieved with both product types.
Features and Benefits
- Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
- Complete protection: Instantly protect your proteins against a broad range of proteases (see Table 1).
- Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
- Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
- Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.
Packaging
Components
Quality
Physical form
Preparation Note
Preparation of Working Solution:
Carefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent breakage of tablets.
One cOmplete Mini tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. The tablets can be added directly to the extraction medium. Alternatively, a stock solution (7 X conc.) can be prepared.
Stock solution (7 X conc.):
Dissolve one cOmplete Mini tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.
Reconstitution
Other Notes
Legal Information
related product
signalword
Warning-Warning
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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