04719956001
Roche
cOmplete™ Lysis-M
storage temp.:room temp
About This Item
Recommended Products
manufacturer/tradename
Roche
storage temp.
room temp
General description
The ready-to-use cOmplete™ Mini Protease Inhibitor Cocktail Tablets, included in the kit, function optimally with the kit′s Lysis-M reagent. The cOmplete™ Mini Protease Inhibitor Tablets allow the inhibition of a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains. cOmplete™ Lysis-M reagent helps in protein extraction and simultaneously inhibit protease activity during the initial extraction steps. cOmplete™ Mini Protease Inhibitor Tablets contain both irreversible and reversible protease inhibitors.
Application
- reporter assays (e.g., β-galactosidase, luciferase, chloramphenicol acetyltransferase)
- immunoassays (e.g., Western blots, ELISAs, RIAs)
- protein assays (e.g., protein kinase A, protein kinase C, and tyrosine kinase)
- protein purification [1]
Note: cOmplete™ Mini tablets (included in the kit) contain EDTA (ethylenediaminetetraacetic acid). If the protein of interest is to be purified by IMAC (immobilized metal-chelate affinity chromatography), e.g., Poly-His tagged recombinant proteins, EDTA has to be removed (e.g., by dialysis) prior to the chromatography. Alternatively, the cOmplete™ Lysis-M EDTA-free product can be used.
Features and Benefits
- Preserves protein structure, since reagents will not denature or otherwise alter proteins.
- Saves money, since one product protects against many proteases.
- Saves steps, since extracted proteins may be used directly in many downstream procedures.
Packaging
Preparation Note
Sample material: Mammalian cells in culture (e.g., Cos-7 cells, harvested at confluency).
Time required: 5 minutes (cell lysis at room temperature).
Number of extractions: 20 (10 ml each).
Effective against: Serine proteases, cysteine proteases, metalloproteases.
Note: Each cOmplete, Mini tablet is concentrated enough to inhibit proteases in 10 ml of extract.
Other Notes
Legal Information
Kit Components Only
- Lysis-M Reagent for protein extraction from mammalian cells
- cOmplete Mini Protease Inhibitor Cocktail Tablets supplied in EASYpacks
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
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cOmplete™ Lysis-M Inhibitor: Low protein concentration in lysate
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