In the presence of a chaotropic salt, RNA binds selectively to special glass fibers prepacked in the High Pure Spin Filter Tube. Bound RNA is purified in a series of rapid wash-and-spin steps to remove contaminating salts, proteins, and other cellular impurities, and then eluted using a low-salt solution. By lowering the concentration of Binding Enhancer during the binding step in the two-column protocol, the small RNA-containing miRNA passes the first column unbound. When the concentration of Binding Enhancer is increased, the small RNA fraction can be bound to a second High Pure Spin Filter Tube.
Low to medium throughput miRNA isolation.
The High Pure miRNA Isolation Kit purifies and enriches small RNAs, such as microRNA (miRNA) from animal cells and tissue samples (including formalin-fixed, paraffin-embedded sections) or plant material. It can also be used to purify total RNA or to prepare samples enriched for small RNAs (<100 nucleotides).
MicroRNAs are natural regulators of gene expression, affecting almost every cellular process (growth and development, cell differentiation, cell death). They occur naturally in diverse animal and plant species, and hundreds have been discovered since the late 1990s. A single miRNA can ramp down expression of multiple genes. Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.
Capacity: The High Pure Spin Filter Tubes hold up to 700 μL sample volume.