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10102105001

Roche

Adenosine Deaminase (ADA)

from calf intestine

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Synonym(s):
ADA
Enzyme Commission number:

biological source

bovine (calf) intestine

Quality Level

form

solution

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

mol wt

230-440 kDa (high)
30-47 kDa (low )

packaging

pkg of 2 mL (10 mg)

manufacturer/tradename

Roche

optimum pH

7.0-7.4

shipped in

wet ice

storage temp.

2-8°C

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A5168A5043P0762
Adenosine Deaminase (ADA) from calf intestine

10102105001

Adenosine Deaminase (ADA)

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

specific activity

150-200 units/mg protein

specific activity

≥130 units/mg protein

specific activity

250-1000 units/mL packed gel

form

solution

form

ammonium sulfate suspension

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

optimum pH

7.0-7.4

optimum pH

-

optimum pH

-

optimum pH

-

mol wt

230-440 kDa (high), 30-47 kDa (low )

mol wt

32.5-33 kDa

mol wt

32.5-33 kDa

mol wt

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

General description

Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Application

Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.
Adenosine deaminase has been used to pre-treat primary Kupffer cells.

Biochem/physiol Actions

Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.
Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Quality

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Physical form

Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6

Preparation Note

Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Dusing MR, et al.
The Journal of biological chemistry, 276(17), 14434-14442 (2001)
Purification and characterization of intestinal adenosine deaminase from mice
Singh L and Sharma R
Molecular and Cellular Biochemistry, 204(1-2), 127-134 (2000)
Regulation of MMP-9 expression by the A2b adenosine receptor and its dependency on TNF-alpha signaling
Chen H, et al.
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Activation of the macrophage A2b adenosine receptor regulates tumor necrosis factor-alpha levels following vascular injury
Chen H, et al.
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