MilliporeSigma
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10104159001

Roche

DNase I

grade II, from bovine pancreas

All Photos(1)
Synonym(s):
Deoxyribonuclease, Deoxyribonucleate 5′-oligonucleotido-hydrolase

biological source

bovine pancreas

Quality Level

100

form

lyophilized

packaging

pkg of 100 mg

manufacturer/tradename

Roche

technique(s)

DNA extraction: suitable

optimum pH

~7.0

storage temp.

2-8°C

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This Item
AMPD11128493200104536282001
DNase I grade II, from bovine pancreas

Roche

10104159001

DNase I

DNase I Amplification Grade

Sigma-Aldrich

AMPD1

DNase I

DNase I from bovine pancreas

Roche

11284932001

DNase I

DNase I recombinant grade I, from bovine pancreas, expressed in Pichia pastoris

Roche

04536282001

DNase I recombinant

biological source

bovine pancreas

biological source

-

biological source

bovine pancreas

biological source

-

form

lyophilized

form

liquid

form

lyophilized

form

lyophilized

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

technique(s)

DNA extraction: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

optimum pH

~7.0

optimum pH

-

optimum pH

-

optimum pH

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.

Application

Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-
  • The isolation of cells from lung, skin and tumor samples
  • Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †

Unit Definition

One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.

Preparation Note

Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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