10104159001

DNase I

grade II, from bovine pancreas

• Synonym(s): Deoxyribonuclease, Deoxyribonucleate 5′-oligonucleotido-hydrolase
• UNSPSC Code: 12352204
• NACRES: NA.54
• Biological source: bovine pancreas

Properties

• biological source: bovine pancreas
• form: lyophilized
• packaging: pkg of 100 mg
• manufacturer/tradename: Roche
• technique(s): DNA extraction: suitable
• optimum pH: ~7.0
• storage temp.: 2-8°C

Description

General description

Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.

Application

Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-

• The isolation of cells from lung, skin and tumor samples
• Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †

Preparation Note

Activator: Ca²⁺ (0.12 mM) in combination with Mg²⁺ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Codes: H317,H334
• Precautionary Statements: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash