MilliporeSigma
All Photos(1)

Documents

10109118001

Roche

Reverse Transcriptase AMV

solution, >50 units/μg protein, suitable for RT-qPCR, suitable for RT-PCR

Sign Into View Organizational & Contract Pricing

Synonym(s):
amv reverse transcriptase
Enzyme Commission number:

form

solution

Quality Level

specific activity

>50 units/μg protein

feature

dNTPs included: no
hotstart: no

packaging

pkg of 1,000 U

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

parameter

42 °C optimum reaction temp.

technique(s)

RT-PCR: suitable
RT-qPCR: suitable

input

purified RNA

detection method

probe-based

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
STR1TRANSRTROTOSRTRO
specific activity

>50 units/μg protein

specific activity

-

specific activity

0.05 U/mg

specific activity

-

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

storage condition

-

storage condition

-

storage condition

-

technique(s)

RT-PCR: suitable, RT-qPCR: suitable

technique(s)

RT-PCR: suitable

technique(s)

RT-PCR: suitable, RT-qPCR: suitable

technique(s)

RT-PCR: suitable, RT-qPCR: suitable

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

General description

Reverse Transcriptase, AMV is a gene product of the RNA genome of avian myeloblastosis virus. The enzymatically active forms of the purified enzyme are α,ββ and αβ. The molecular weight of the α-subunit is 68 kDa, that of the β-subunit 92 kDa. The mature αβ form, the most active form of Reverse Transcriptase, AMV, includes a RNA-directed DNA polymerase, a DNA-dependent DNA polymerase, a RNase H, and an unwinding activity. Reverse Transcriptase, AMV is used for cDNA synthesis, for synthesis of first strand cDNA for use in subsequent amplification reactions and dideoxy DNA sequencing.
The enzyme can also be used for RNA sequencing, 3′ end labeling of DNA fragments, and the generation of ss probes for genomic footprints.

Reverse Transcriptase AMV requires a primer and Mg2+ or Mn2+ for activity.

Specificity

AMV (Avian Myeloblastosis Virus) Reverse Transcriptase is an RNA-directed DNA polymerase and a DNA-dependent DNA polymerase. The AMV reverse transcriptase requires a primer and Mg2+ or Mn2+ for activity.
Heat inactivation: 5 min, 95 °C

Application

Reverse Transcriptase AMV is suitable for:
  • First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)
  • Dideoxy DNA sequencing
  • Primer extension
  • RNA sequencing
  • 3′-end labeling of DNA fragments
  • Generation of single-stranded probes for genomic footprint experiments

Features and Benefits

  • Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
  • Procure full length cDNA fragments up to 12 kb
  • Higher thermostability (up to 60°C) and specificity than M-MuLV Reverse Transcriptase

Packaging

1 kit containing 2 components

Quality

Absence of contaminants: Tested for the absence of detectable nonspecific RNases and nonspecific DNases in incubations with various nucleic acids (analyzed by gel electrophoresis) according to the current Quality Control procedures.
Function test: Reverse Transcriptase AMV is function tested in the cDNA Synthesis Kit and RT-PCR.

Unit Definition

1 unit is the enzyme activity that incorporates 1 nmol of [3H]-dTMP into acid-precipitable products in 10 minutes at +37 °C with poly(A)+ · d(pT)15 as template primer.

Volume Activity: 20-25 U/μl. Please refer to the Certificate of Analysis for more information.

Preparation Note

Storage buffer: 200 mM potassium phosphate, 2 mM dithiothreitol, 0.2% Triton X-100 (v/v), 50% glycerol (v/v), pH 7.2.

Storage and Stability

Store at -15–-25 °C. (Storage at -70 °C is more likely to deteriorate the enzyme via repeated freezing and thawing.)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Reverse Transcriptase AMV 20-25 U/μl

  • First-strand cDNA Synthesis Buffer 5x concentrated

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

Lucile Pantel et al.
Molecular cell, 70(1), 83-94 (2018-04-07)
Growing resistance of pathogenic bacteria and shortage of antibiotic discovery platforms challenge the use of antibiotics in the clinic. This threat calls for exploration of unconventional sources of antibiotics and identification of inhibitors able to eradicate resistant bacteria. Here we
Crystal M Gigante et al.
Viruses, 12(11) (2020-11-08)
As countries with endemic canine rabies progress towards elimination by 2030, it will become necessary to employ techniques to help plan, monitor, and confirm canine rabies elimination. Sequencing can provide critical information to inform control and vaccination strategies by identifying
Maternal licking regulates hippocampal glucocorticoid receptor transcription through a thyroid hormone-serotonin-NGFI-A signalling cascade.
Hellstrom I C, et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 367(1601), 2495-2510 (2012)
Mélanie A Cron et al.
Journal of neuroinflammation, 17(1), 294-294 (2020-10-10)
Myasthenia gravis (MG) is a rare autoimmune disease mainly mediated by autoantibodies against the acetylcholine receptor (AChR) at the neuromuscular junction. The thymus is the effector organ, and its removal alleviates the symptoms of the disease. In the early-onset form
Anirudh Chakravarthy et al.
Life science alliance, 4(12) (2021-10-02)
The continued resurgence of the COVID-19 pandemic with multiple variants underlines the need for diagnostics that are adaptable to the virus. We have developed toehold RNA-based sensors across the SARS-CoV-2 genome for direct and ultrasensitive detection of the virus and

Related Content

RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service