10109118001

Roche

Reverse Transcriptase AMV

Synonym(s):
amv reverse transcriptase
Enzyme Commission number:

form

solution

Quality Level

specific activity

>50 units/μg protein

packaging

pkg of 1,000 U

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

parameter

42 °C optimum reaction temp.

shipped in

dry ice

General description

Reverse Transcriptase, AMV is a gene product of the RNA genome of avian myeloblastosis virus. The enzymatically active forms of the purified enzyme are α,ββ and αβ. The molecular weight of the α-subunit is 68 kDa, that of the β-subunit 92 kDa. The mature αβ form, the most active form of Reverse Transcriptase, AMV, includes a RNA-directed DNA polymerase, a DNA-dependent DNA polymerase, a RNase H, and an unwinding activity. Reverse Transcriptase, AMV is used for cDNA synthesis, for synthesis of first strand cDNA for use in subsequent amplification reactions and dideoxy DNA sequencing.
The enzyme can also be used for RNA sequencing, 3′ end labeling of DNA fragments, and the generation of ss probes for genomic footprints.

Reverse Transcriptase AMV requires a primer and Mg2+ or Mn2+ for activity.

Specificity

AMV (Avian Myeloblastosis Virus) Reverse Transcriptase is an RNA-directed DNA polymerase and a DNA-dependent DNA polymerase. The AMV reverse transcriptase requires a primer and Mg2+ or Mn2+ for activity.
Heat inactivation: 5 min, 95 °C

Application

Reverse Transcriptase AMV is suitable for:
  • First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)
  • Dideoxy DNA sequencing
  • Primer extension
  • RNA sequencing
  • 3′-end labeling of DNA fragments
  • Generation of single-stranded probes for genomic footprint experiments

Features and Benefits

  • Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
  • Procure full length cDNA fragments up to 12 kb
  • Higher thermostability (up to 60°C) and specificity than M-MuLV Reverse Transcriptase

Packaging

1 kit containing 2 components

Quality

Absence of contaminants: Tested for the absence of detectable nonspecific RNases and nonspecific DNases in incubations with various nucleic acids (analyzed by gel electrophoresis) according to the current Quality Control procedures.
Function test: Reverse Transcriptase AMV is function tested in the cDNA Synthesis Kit and RT-PCR.

Unit Definition

1 unit is the enzyme activity that incorporates 1 nmol of [3H]-dTMP into acid-precipitable products in 10 minutes at +37 °C with poly(A)+ · d(pT)15 as template primer.

Volume Activity: 20-25 U/μl. Please refer to the Certificate of Analysis for more information.

Preparation Note

Storage buffer: 200 mM potassium phosphate, 2 mM dithiothreitol, 0.2% Triton X-100 (v/v), 50% glycerol (v/v), pH 7.2.

Storage and Stability

Store at -15–-25 °C. (Storage at -70 °C is more likely to deteriorate the enzyme via repeated freezing and thawing.)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Reverse Transcriptase AMV 20-25 U/μl

  • First-strand cDNA Synthesis Buffer 5x concentrated

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Lucile Pantel et al.
Molecular cell, 70(1), 83-94 (2018-04-07)
Growing resistance of pathogenic bacteria and shortage of antibiotic discovery platforms challenge the use of antibiotics in the clinic. This threat calls for exploration of unconventional sources of antibiotics and identification of inhibitors able to eradicate resistant bacteria. Here we...
Crystal M Gigante et al.
Viruses, 12(11) (2020-11-08)
As countries with endemic canine rabies progress towards elimination by 2030, it will become necessary to employ techniques to help plan, monitor, and confirm canine rabies elimination. Sequencing can provide critical information to inform control and vaccination strategies by identifying...
Maternal licking regulates hippocampal glucocorticoid receptor transcription through a thyroid hormone-serotonin-NGFI-A signalling cascade.
Hellstrom I C, et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 367(1601), 2495-2510 (2012)
Jessica N Saykally et al.
PloS one, 4(12), e8460-e8460 (2009-12-31)
Four genome-wide association studies mapped an "obesity" gene to human chromosome 10p11-12. As the zinc finger E-box binding homeobox 1 (ZEB1) transcription factor is encoded by the TCF8 gene located in that region, and as it influences the differentiation of...
Mélanie A Cron et al.
Journal of neuroinflammation, 17(1), 294-294 (2020-10-10)
Myasthenia gravis (MG) is a rare autoimmune disease mainly mediated by autoantibodies against the acetylcholine receptor (AChR) at the neuromuscular junction. The thymus is the effector organ, and its removal alleviates the symptoms of the disease. In the early-onset form...
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