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MilliporeSigma

10109134001

Roche

RNase

from bovine pancreas

Synonym(s):

Rnase

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500 MG

$194.00

$194.00

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
Specific activity:
~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)
Biological source:
bovine pancreas

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biological source

bovine pancreas

form

dry powder (prepared from crystallized ribonuclease)

specific activity

~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)

packaging

pkg of 500 mg

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

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1 of 4

This Item
83834R5500R5250
RNase from bovine pancreas

Roche

10109134001

RNase

Ribonuclease A from bovine pancreas 4×cryst., ~70 U/mg (acc. to Kunitz)

Sigma-Aldrich

83834

Ribonuclease A from bovine pancreas

Ribonuclease A from bovine pancreas Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein

Sigma-Aldrich

R5500

Ribonuclease A from bovine pancreas

Ribonuclease A from bovine pancreas Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

Sigma-Aldrich

R5250

Ribonuclease A from bovine pancreas

biological source

bovine pancreas

biological source

-

biological source

bovine pancreas

biological source

bovine pancreas

specific activity

~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)

specific activity

~70 U/mg (acc. to Kunitz)

specific activity

75-125 Kunitz units/mg protein

specific activity

≥70 Kunitz units/mg protein

form

dry powder (prepared from crystallized ribonuclease)

form

powder

form

lyophilized powder

form

buffered aqueous solution

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

300

Quality Level

200

General description

Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).
Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.[1]

Application

RNase has been used in DNA ladder assay.[2]
RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis.[3] It has been used during flow cytometry analysis.[4]

Biochem/physiol Actions

Heat inactivation: 1 mM EDTA

Preparation Note

Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.

Other Notes

For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
69994920
64544420
62670720
58538720
49703521

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Ribonucleases (2011)
S Saito et al.
The Journal of biological chemistry, 275(44), 34528-34533 (2000-08-10)
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Molecular biology of the cell, 14(7), 2844-2860 (2003-07-15)
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Methods in molecular biology (Clifton, N.J.), 1476, 143-165 (2016-08-11)
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