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| Size/SKU | Availability | Price |
|---|---|---|
50 mg | Available to ship TODAYfromMILWAUKEE | $80.10 |
About This Item
UNSPSC Code:
12352207
NACRES:
NA.54
$80.10
Available to ship TODAYDetails
biological source
soybean
form
lyophilized
mol wt
Mr 20,100
packaging
pkg of 50 mg
manufacturer/tradename
Roche
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
pH
3.8-4.5
solubility
water: soluble
storage temp.
2-8°C
General description
Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.
Application
Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
- in the buffer for hepatocyte isolation
- to inhibit trypsin in Corneal epithelial cells
- to inhibit papain activity while treating hippocampal neural stem cells (NSCs)
Biochem/physiol Actions
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.
Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.
Features and Benefits
Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)
Isoelectric point: 4.5
Isoelectric point: 4.5
Preparation Note
Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
Sensitive to heat, alkaline pH, and protein-precipitating compounds.
Store at 2 to 8 °C. (Store dry!)
Other Notes
1 U = 270 S & T-units or 90 USP-units
For life science research only. Not for use in diagnostic procedures.
1 of 1
This Item | |||
|---|---|---|---|
| biological source soybean | biological source Glycine max (soybean) | biological source chicken egg white | biological source Glycine max (soybean) |
| form lyophilized | form lyophilized powder | form powder | form lyophilized powder |
| solubility water: soluble | solubility - | solubility water: soluble | solubility 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL, clear, colorless to very faintly yellow |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt Mr 20,100 | mol wt - | mol wt - | mol wt 20 kDa |
| concentration 1 mg/mL | concentration - | concentration 1 mg/mL | concentration - |
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Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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