10165948001

RNA, MS2

from bacteriophage MS2

• Synonym(s): RNA, ribonucleic acid
• UNSPSC Code: 41106311
• NACRES: NA.54
• Biological source: bacteriophage MS2
• Form: solution
• Solubility: water: miscible
• Storage temp.: −20°C (−15°C to −25°C)

Properties

• biological source: bacteriophage MS2
• form: solution
• mol wt: ~1200 kDa
• packaging: pkg of 500 μL (10 A₂₆₀ units)
• manufacturer/tradename: Roche
• technique(s): RNA extraction: suitable
• color: colorless
• solubility: water: miscible
• storage temp.: −20°C (−15°C to −25°C)

Description

General description

RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus.[1]

Application

RNA, MS2 has been used:

• as a standard to assess RNA quantification methods[2]
• in RNA extraction[3][4]
• in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types[5]
• for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis.
• for structural and functional studies.
• to stabilize RNA during cDNA synthesis[2]
• to improve RNA yield during RNA extraction or isolation[6][7][4]

Preparation Note

Working solution: Storage buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.0.

Analysis Note

Typical analysis: Free of protein and host nucleic acids according to current Quality Control procedures.

Other Notes

Chain Length 3,569 nucleotides

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: does not flash
• flash_point_c: does not flash