MilliporeSigma
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10165948001

Roche

RNA, MS2

from bacteriophage MS2

Synonym(s):
RNA, ribonucleic acid

Quality Level

form

solution

mol wt

~1200 kDa

packaging

pkg of 500 μL (10 A260 units)

manufacturer/tradename

Roche

technique(s)

RNA extraction: suitable

shipped in

dry ice

storage temp.

−20°C (−15°C to −25°C)

General description

RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus.

Application

RNA, MS2 has been used:
  • as a standard to assess RNA quantification methods
  • in RNA extraction
  • in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types
  • for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis.
  • for structural and functional studies.
  • to stabilize RNA during cDNA synthesis
  • to improve RNA yield during RNA extraction or isolation

Quality

Typical analysis: Free of protein and host nucleic acids according to current Quality Control procedures.

Sequence

Chain Length 3,569 nucleotides

Preparation Note

Working solution: Storage buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.0.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Michael Eikmans et al.
International journal of molecular sciences, 21(11) (2020-06-12)
About 10-15% of couples who want to conceive suffer from subfertility, while in 30% of these cases, a male factor plays a role. Levels of particular microRNAs in seminal plasma, including those involved in spermatogenesis, may serve as an indicative
Shalini Das Gupta et al.
Scientific reports, 10(1), 9012-9012 (2020-06-04)
Quantification of plasma microRNAs (miRNAs) as non-invasive disease biomarkers is subject to multiple technical variabilities. This study aimed to develop an optimized protocol for miRNA quantification from rodent plasma. We hypothesized that a fixed small RNA concentration input for reverse
Petra Vychytilova-Faltejskova et al.
Carcinogenesis, 37(10), 941-950 (2016-08-04)
Early detection of colorectal cancer is the main prerequisite for successful treatment and reduction of mortality. Circulating microRNAs were previously identified as promising diagnostic, prognostic and predictive biomarkers. The purpose of this study was to identify serum microRNAs enabling early
Aitana Braza-Boïls et al.
International journal of molecular sciences, 21(8) (2020-04-25)
There is evidence for the effects of platelet inhibition on innate immune activation. Circulating microRNAs (miRNAs) have been implicated as markers of platelet and leukocyte activation. In the present study, we assessed the effects of P2Y12 inhibitors on platelet and
Rom S Leidner et al.
PloS one, 8(3), e57841-e57841 (2013-03-09)
Genome-wide platforms for high-throughput profiling of circulating miRNA (oligoarray or miR-Seq) offer enormous promise for agnostic discovery of circulating miRNA biomarkers as a pathway for development in breast cancer detection. By harmonizing data from 15 previous reports, we found widespread

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