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10197734001

Roche

Glutamate Dehydrogenase (GlDH)

from beef liver

Synonym(s):

DNA marker

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Pack SizeSKUAvailabilityPrice
3000 units

Estimated to ship onJune 09, 2026fromMILWAUKEE

$129.00

About This Item

UNSPSC Code:
12352204
EC Number:
1.4.1.3 (BRENDA, IUBMB)
NACRES:
NA.54
Specific activity:
10 U/mg, ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
Biological source:
bovine liver
Concentration:
≥10-20 % (w/w)

$129.00

Estimated to ship onJune 09, 2026Details

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biological source

bovine liver

form

lyophilized

specific activity

10 U/mg, ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)

packaging

pkg of 3,000 U

manufacturer/tradename

Roche

concentration

≥10-20 % (w/w)

technique(s)

activity assay: suitable

color

white

optimum pH

8

solubility

water: 20 mg/mL, colorless

suitability

suitable for UV spectrophotometry and general use

UniProt accession no.

P00366

application(s)

life science and biopharma

foreign activity

ADH <0.00500%, LDH <0.00500%, MDH <0.00500%

shipped in

wet ice

storage temp.

2-8°C

Gene Information

bovine ... GLUD1(281785)

General description

Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.
GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria.[1] It acts as a branch-point enzyme between amino acid oxidation and urea production.[2] L-glutamate:NAD(P)+ oxidoreductase is involved in deamination.

Application

Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.

Analysis Note

Contaminants: <0.005% ADH, LDH, and MDH, each

Other Notes

For life science research only. Not for use in diagnostic procedures.
The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD).

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Glutamate Dehydrogenase (GlDH) from beef liver

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Glucose-6-Phosphate Dehydrogenase (G6P-DH)

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&#946;-Galactose Dehydrogenase S suspension, ~80&#160;units/mg protein (At 25 °C with galactose as the substrate.), pkg of 50&#160;U

Roche

10662046001

β-Galactose Dehydrogenase S

specific activity

10 U/mg, ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)

specific activity

~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)

specific activity

~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)

specific activity

80 U/mg (Approximately 80 U/mg protein at +25°C with galactose as the substrate.), ~80 units/mg protein (At 25 °C with galactose as the substrate.)

biological source

bovine liver

biological source

bacterial (Leuconostoc mesenteroides)

biological source

yeast

biological source

-

Gene Information

bovine ... GLUD1(281785)

Gene Information

-

Gene Information

Saccharomyces cerevisiae ... ZWF1(855480)

Gene Information

-

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

-

form

lyophilized

form

solution, suspension

form

suspension

form

suspension

application(s)

life science and biopharma

application(s)

life science and biopharma

application(s)

life science and biopharma

application(s)

-

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
84117722
81204523
74453425
74453424
65527323

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Disruption of the cell wall integrity gene ECM33 results in improved fermentation by wine yeast
Jin Zhang
Metabolic engineering (2018)
G Hughes et al.
Analytica chimica acta, 933, 82-88 (2016-08-09)
A reagentless glutamate biosensor was applied to the determination of glutamate released from liver hepatocellular carcinoma cells (HepG2) in response to toxic challenge from various concentrations of paracetamol. A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola's Blue (MB-SPCE)
T J Stillman et al.
Journal of molecular biology, 234(4), 1131-1139 (1993-12-20)
We have solved the structure of the binary complex of the glutamate dehydrogenase from Clostridium symbiosum with glutamate to 1.9 A resolution. In this complex, the glutamate side-chain lies in a pocket on the enzyme surface and a key determinant
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Global Trade Item Number

SKUGTIN
1019773400104061838668707