10220647001

GTP-γ-S

Tetralithium salt

• Synonym(s): GTP-γ-S, GTP
• UNSPSC Code: 12352204
• NACRES: NA.54
• Assay: 87% (HPLC)
• Form: solid
• Solubility: water: soluble
• Storage temp.: −20°C (−15°C to −25°C)

Properties

• description: C₁₀H₁₂N₅O₁₃P₃SLi₄
• assay: 87% (HPLC)
• form: solid
• mol wt: M_r 539.2 (GTP-γ-S), M_r 563.0 (GTP-γ-S-Li₄)
• composition: GDP, ≤12% HPLC , GMP, ≤1% HPLC , GTP, ≤2% HPLC
• packaging: pkg of 10 mg
• manufacturer/tradename: Roche
• storage condition: protect from light
• concentration: 97%
• technique(s): activity assay: suitable
• color: white
• solubility: water: soluble
• storage temp.: −20°C (−15°C to −25°C)

Description

General description

Guanosine 5′-O-(3-thiotriphosphate), tetralithium salt (GTP-γ-S) is a non-hydrolysable analog of GTP.[1] It is an inhibitor of phosphodiesterase in photoreceptors.[2]

Application

GTP-γ-S has been used:

• in G-Protein activation assay to assess the functionality of protease-activated receptor 4 (PAR4)[3]
• in fluorimetric guanine nucleotide exchange assay of G protein alpha subunits (Gα)[4]
• to monitor the Rac family small guanosine triphosphatase (GTPase) 2 (Rac2)-stimulated activity of phospholipase Cγ2 mutants[5]

Biochem/physiol Actions

Guanosine 5′-O-(3-thiotriphosphate) (GTP-γ-S) activates guanine-nucleotide-binding proteins and is slowly hydrolyzed enzymatically.[6] It inhibits guanosine triphosphatase (GTPases) more potently than guanosine triphosphate (GTP),

Preparation Note

Store powder dry at -15 to -25 °C; aqueous solutions with pH approx. 7 should be stable for six months at -15 to -25 °C.

Analysis Note

Absorption: Content is measured in aqueous solution by absorption at 254 nm.
Conditions:

• approx. 10 mg/ml double-dist. water
• dilution: 9.8 ml double-dist. water + 0.2 ml sample.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash