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10295892001

Roche

Colcemid

solution, suitable for blocking, sterile; 0.2 μm filtered

Synonym(s):

Demecolcine, N-Deacetyl-N-methylcolchicine

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Size/SKUAvailabilityPrice
20 mL

Available to ship TODAYfromMILWAUKEE

$66.10

About This Item

Empirical Formula (Hill Notation):
C21H25NO5
CAS Number:
477-30-5
Molecular Weight:
371.43
PubChem Substance ID:
329749009
UNSPSC Code:
12352207
Beilstein/REAXYS Number:
2822892
MDL number:
MFCD00075459
NACRES:
NA.54
Form:
solution

$66.10

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description

N-methyl-N-deacetyl-colchicine

sterility

sterile; 0.2 μm filtered

form

solution

packaging

pkg of 20 mL (10 μg/ml)

manufacturer/tradename

Roche

technique(s)

blocking: suitable

impurities

microbial, tested

solubility

water: miscible

storage temp.

2-8°C

SMILES string

CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13

InChI

1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1

InChI key

NNJPGOLRFBJNIW-HNNXBMFYSA-N

General description

Colcemid is also known as demecolcine. Its generic name is N-methyl-N-deacetyl-colchicine. Colcemid depolymerizes microtubules and blocks mitosis at metaphase.[1]

Application

Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis.[2][3]

Biochem/physiol Actions

Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.

Physical form

Solution (10 μg/ml), filtered through 0.2 μm pore size membrane.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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This Item
234109-MD1925D7385
Colcemid solution, suitable for blocking, sterile; 0.2 μm filtered

Roche

10295892001

Colcemid

Colcemid Cell synchronization agent.

Millipore

234109-M

Colcemid

Demecolcine solution 10 μg/mL in HBSS, ACF Qualified, BioXtra

Sigma-Aldrich

D1925

Demecolcine solution

Demecolcine ≥98% (HPLC)

Sigma-Aldrich

D7385

Demecolcine

form

solution

form

crystalline powder

form

liquid

form

powder

technique(s)

blocking: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

manufacturer/tradename

Roche

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

2-8°C

storage temp.

2-8°C

solubility

water: miscible

solubility

chloroform: 10 mg/mL, ethanol: soluble

solubility

-

solubility

chloroform: 10 mg/mL, ethanol: soluble

impurities

microbial, tested

impurities

-

impurities

-

impurities

-

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

does not flash

flash_point_c

does not flash

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