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10354074001

Roche

Citrate Lyase (CL)

from Klebsiella pneumoniae (Aerobacter aerogenes)

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Synonym(s):
Citrate Lyase (CL)
Enzyme Commission number:

biological source

bacterial (Klebsiella pneumoniae)

Quality Level

form

lyophilized

specific activity

≥0.25 U/mg (At 25 °C with citrate as the substrate.)

packaging

pkg of 120 U

manufacturer/tradename

Roche

technique(s)

activity assay: suitable

color

light cream

optimum pH

8.0-9.0

solubility

water: soluble

NCBI accession no.

UniProt accession no.

application(s)

life science and biopharma

foreign activity

ICDH <0.05% (NAD as coenzyme)
NADH oxidase <0.05%

storage temp.

2-8°C

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This Item
C0897ABC426SAB4501964
vibrant-m

10354074001

Citrate Lyase (CL)

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:5000, western blot: 1:500-1:1000

biological source

bacterial (Klebsiella pneumoniae)

biological source

-

biological source

rabbit

biological source

rabbit

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

NCBI accession no.

WP_323133469

NCBI accession no.

-

NCBI accession no.

NP_001087

NCBI accession no.

P53396

optimum pH

8.0-9.0

optimum pH

-

optimum pH

-

optimum pH

-

General description

Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate.

Application

It was used in enzymatic assay to measure citrate concentration using microtiter plates.

Quality

Contaminants: <0.05% ICDH (NAD specific) and “NADH oxidase”, each

Physical form

Lyophilizate, stabilized with BSA, sucrose, MgSO4 and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized.

Preparation Note

Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Agata M Pudlik et al.
Journal of bacteriology, 193(3), 706-714 (2010-12-01)
Carbohydrate/citrate cometabolism in Lactococcus lactis results in the formation of the flavor compound acetoin. Resting cells of strain IL1403(pFL3) rapidly consumed citrate while producing acetoin when substoichiometric concentrations of glucose or l-lactate were present. A proton motive force was generated

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