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10567752001

Roche

Alkaline Phosphatase

EIA grade, from calf intestine

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Synonym(s):
AP, Alkaline Phosphatase
Enzyme Commission number:

biological source

bovine (calf) intestine

Quality Level

form

solution

specific activity

~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)

packaging

pkg of 1.5 mL (15 mg)

manufacturer/tradename

Roche

optimum pH

9.8

pH range

9.0-11.0

shipped in

wet ice

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This Item
P0762P5521P7923
Alkaline Phosphatase EIA grade, from calf intestine

Roche

10567752001

Alkaline Phosphatase

form

solution

form

ammonium sulfate suspension

form

aqueous solution

form

buffered aqueous glycerol solution

specific activity

~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)

specific activity

250-1000 units/mL packed gel

specific activity

≥2,000 DEA units/mg protein

specific activity

≥4,000 DEA units/mg protein

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

optimum pH

9.8

optimum pH

-

optimum pH

-

optimum pH

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

General description

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Amino groups: 8-16 moles/mole enzyme

Application

Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis.

Quality

Purity: single peak (HPLC)

Sequence

Purification procedure
AP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol.
In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in AP
No information in literature about -SH groups in AP.

Carbohydrate structure
Results of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available.

NH2- and carbohydrate estimation
Results of NH2- and carbohydrate estimation are available.

Unit Definition

Unit Conversion: Approx. 4.35 U (EIA grade AP) [37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5]
1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions]

Physical form

Solution, ready-to-use, 10mg/ml

Preparation Note

Storage conditions (working solution): The diluted enzyme (1:10 in 3 M NaCl) will give a stability of 6 months at 4 °C.
1:10 dilution in water will give stability for 1 week at 4°C.
Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity.

Storage and Stability

Store at 2 to 8 °C. (Do not freeze)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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Yupeng Li et al.
Chemical research in toxicology, 34(4), 992-1003 (2021-03-12)
The tobacco-specific nitrosamines N'-nitrosonornicotine (NNN) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) are considered to be two of the most important carcinogens in unburned tobacco and its smoke. They readily cause tumors in laboratory animals and are classified as "carcinogenic to humans" by the
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Huntington's disease (HD) is a fatal, dominantly inherited neurodegenerative disorder caused by CAG trinucleotide expansion in exon 1 of the huntingtin (HTT) gene. Since the reduction of pathogenic mutant HTT messenger RNA is therapeutic, we developed a mutant allele-sensitive CAGEX

Protocols

Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.

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