Roche

Thrombin

Name: Thrombin
Brand: ROCHE
Price: 223 USD
Availability: InStock

from human plasma

Synonym(s):

coagulation factor Iia, thrombin

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20 UNITS

$223.00

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About This Item

UNSPSC Code:

12352204

EC Number:

3.4.21.5 (BRENDA, IUBMB)

Specific activity:

~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.)

Biological source:

human plasma

Key Documents

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biological source

human plasma

form

lyophilized

specific activity

~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.)

mol wt

M_r ~33.6 kDa

packaging

pkg of 20 U

manufacturer/tradename

Roche

optimum pH

8.2-9.0

shipped in

wet ice

Related Categories

Albumins and Transport Proteins

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Molecular Biology & Functional Genomics

Proteins & Enzymes

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Show Differences

1 of 4

This Item	1.12374	927074	SRP6557
Roche 10602400001 Thrombin	Sigma-Aldrich 1.12374 Thrombin	Sigma-Aldrich 927074 TissueFab^® bioink kit	Sigma-Aldrich SRP6557 Thrombin Active from human plasma
biological source human plasma	biological source bovine plasma	biological source -	biological source human
specific activity ~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.)	specific activity 50 NIH units/mg dry wt	specific activity -	specific activity -
form lyophilized	form solid	form (Solid chunks, fibers or powder)	form lyophilized
mol wt M_r ~33.6 kDa	mol wt -	mol wt -	mol wt 37 kDa
shipped in wet ice	shipped in -	shipped in -	shipped in wet ice
optimum pH 8.2-9.0	optimum pH -	optimum pH -	optimum pH -

General description

Thrombin is a Na⁺ activated allosteric serine protease. It belongs to chymotrypsin family.[1] Thrombin is made of two polypeptide chains of 36 (A chain) and 259 (B chain) residues that are covalently attached with a disulfide bond.[1]

Thrombin is a coagulation factor IIa.[2] It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of Arg.[3] The enzyme converts fibrinogen to fibrin.[4] The product contains EDTA and additional stabilizing agents.

Application

Thrombin has been used in the expression and purification of recombinant RELM (resistin-like moleculefamily members.[5] It has also been used in platelet spreading on fibrinogen[6][7] and immunostaining.[8]

Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.[8][9]

Biochem/physiol Actions

Thrombin plays an important role in blood coagulation. It serve as a procoagulant factor while transforming fibrinogen into an insoluble fibrin clot. Thrombin can act as anticoagulant by the activation of protein C.[1]

Physical form

Lyophilizate

Preparation Note

Storage conditions (working solution): -15 to -25°C

Inhibitors: DFP, TLCK, PMSF, benzamidine, α₁-antitrypsin, α₂-macroglobulin, antithrombin III heparin, hirudin, and APMSF

Store at 2 to 8 °C. (Store dry!)

The reconstituted solution contains 20 mM EDTA.
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!

Other Notes

For life science research only. Not for use in diagnostic procedures.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

pictograms

GHS07,GHS08

signalword

Warning

hcodes

H332,H373

pcodes

P260 - P271 - P304 + P340 + P312 - P314 - P501

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash

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Partially defective store operated calcium entry and Hem (ITAM) signaling in platelets of serotonin transporter deficient Mice.

Wolf K, et al.

PLoS ONE, 11(1), e0147664-e0147664 (2016)

Thrombin: Physiology and Disease (2010)

Characterizing thrombus with multiple red blood cell compositions by optical coherence tomography attenuation coefficient.

Hsiao-Chuan Liu et al.

Journal of biophotonics, 14(3), e202000364-e202000364 (2020-12-15)

Embolectomy is one of the emergency procedures performed to remove emboli. Assessing the composition of human blood clots is an important diagnostic factor and could provide guidance for an appropriate treatment strategy for interventional physicians. Immunostaining has been used to

Dynamic reconfiguration of pro-apoptotic BAK on membranes.

Jarrod J Sandow et al.

The EMBO journal, 40(20), e107237-e107237 (2021-09-16)

BAK and BAX, the effectors of intrinsic apoptosis, each undergo major reconfiguration to an activated conformer that self-associates to damage mitochondria and cause cell death. However, the dynamic structural mechanisms of this reconfiguration in the presence of a membrane have

Fibrinogen and fibrin structure and functions.

M W Mosesson

Journal of thrombosis and haemostasis : JTH, 3(8), 1894-1904 (2005-08-17)

Fibrinogen molecules are comprised of two sets of disulfide-bridged Aalpha-, Bbeta-, and gamma-chains. Each molecule contains two outer D domains connected to a central E domain by a coiled-coil segment. Fibrin is formed after thrombin cleavage of fibrinopeptide A (FPA)

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