10775835001

Bovine Serum Albumin

98.5% (electrophoresis), Fatty acids (total) ≤0.2 mg/g, Triglycerides (enzym.) free, Immunoglobulins not detectable, microbiological culture: suitable, USA origin

• Synonym(s): BSA, Bovine Serum Albumin Fraction V, fatty acid free, Bovine albumin, bovine serum
• UNSPSC Code: 12352106
• NACRES: NA.54
• Biological source: bovine
• Assay: 98.5% (electrophoresis)
• Form: lyophilized
• Technique(s): immunofluorescence: suitable, ligand binding assay: suitable
• Impurities: Immunoglobulins, none detected, Triglycerides, none detected, ≤0.2 mg/g fatty acids

Properties

• biological source: bovine
• assay: 98.5% (electrophoresis)
• form: lyophilized
• mol wt: M_r 68 kDa
• packaging: pkg of 50 g
• manufacturer/tradename: Roche
• origin: USA origin
• technique(s): immunofluorescence: suitable, ligand binding assay: suitable
• impurities: Immunoglobulins, none detected, Triglycerides, none detected, ≤0.2 mg/g fatty acids
• UniProt accession no.: P02769
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

General description

Highest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.

Application

Bovine Serum Albumin, Fraction V, fatty acid free is used:

• in lipid research to study lipid metabolism in biological systems[1]
• to identify transport mechanisms in living organisms
• to study ligand binding
• to investigate ionic binding
• in the preparation of solutions during isolation of cardiomyocytes[2]
• as a blocking solution to incubate embryos for immunofluorescence
• in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers

Biochem/physiol Actions

The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.[3]

Features and Benefits

• Free from fatty acids according to current quality control procedures (suitable for lipid research)
• High quality
• Low content of heavy metal ions
• Long-term stability

Analysis Note

Purity: ≥98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable