10786357001
Roche
Ribonuclease H (RNase H)
from Escherichia coli H 560 pol A1
Recommended Products
biological source
Escherichia coli ( H 560 pol A1)
Quality Level
assay
100%
form
solution
specific activity
~40000 units/mg protein
packaging
pkg of 100 U
manufacturer/tradename
Roche
technique(s)
cDNA synthesis: suitable
color
colorless
optimum pH
7.5-9.1
solubility
water: miscible
suitability
suitable for molecular biology
NCBI accession no.
application(s)
life science and biopharma
foreign activity
RNase, none detected (up to 10 U with MS- II- RNA)
endonuclease ~10 units, none detected (using lambda-DNA)
nicking activity 10 units, none detected
shipped in
dry ice
storage temp.
−20°C (−15°C to −25°C)
Gene Information
Escherichia coli ... rnhA(946955)
General description
Source: E. coli H560 pol A1
Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)
Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.
Application
- In vivo RNA-primed initiation of DNA synthesis
- Elimination of mRNA during second-strand cDNA synthesis
- Site-specific cleavage of RNA
- Detection of RNA:DNA regions in double-stranded DNA of natural origin
- Removal of poly (A) sequences of mRNA if oligo (dT) is present
- RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)
Biochem/physiol Actions
Features and Benefits
- Eliminate potential sources of PCR errors.
- Increase accessibility of primers during subsequent PCR.
Quality
Unit Definition
Volume Activity: Approximately 1 U/μl
Preparation Note
Storage and Stability
Other Notes
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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