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10791156001

Roche

Endoproteinase Glu-C (V8 Protease)

from Staphylococcus aureus V8

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Synonym(s):
V8 protease, protease v8
Enzyme Commission number:
3.4.21.19 (BRENDA, IUBMB)

form

lyophilized (salt-free)

specific activity

20 U/mg (Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).)
~20 units/mg protein (At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).)

mol wt

30 kDa

packaging

pkg of 2 mg

manufacturer/tradename

Roche

optimum pH

8.0-8.5

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This Item
ENDOGLUS-RO1019773400110662046001
Endoproteinase Glu-C (V8 Protease) from Staphylococcus aureus V8

Roche

10791156001

Endoproteinase Glu-C (V8 Protease)

Endoproteinase Glu-C Sequencing Grade from Staphylococcus aureus V8

Roche

ENDOGLUS-RO

Endoproteinase Glu-C Sequencing Grade

Glutamate Dehydrogenase (GlDH) from beef liver

Roche

10197734001

Glutamate Dehydrogenase (GlDH)

β-Galactose Dehydrogenase S suspension, ~80 units/mg protein (At 25 °C with galactose as the substrate.), pkg of 50 U

Roche

10662046001

β-Galactose Dehydrogenase S

specific activity

20 U/mg (Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).), ~20 units/mg protein (At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).)

specific activity

≥15 units/mg protein

specific activity

10 U/mg (Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.), ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)

specific activity

80 U/mg (Approximately 80 U/mg protein at +25°C with galactose as the substrate.), ~80 units/mg protein (At 25 °C with galactose as the substrate.)

mol wt

30 kDa

mol wt

Mr 27 kDa

mol wt

-

mol wt

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

optimum pH

8.0-8.5

optimum pH

4.0-7.8

optimum pH

8

optimum pH

-

packaging

pkg of 2 mg

packaging

pkg of 3 × 50 μg (11047817001), pkg of 50 μg (11420399001)

packaging

pkg of 3,000 U

packaging

pkg of 50 U

General description

Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK.

Specificity

Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.

Application

Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.

Biochem/physiol Actions

Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.

Preparation Note

Activator: The enzyme has its maximal activity in presence of SH-reagents
Working concentration: 1 to 5 mM
Working solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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324796

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Sigma-Aldrich

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Sigma-Aldrich

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