10843555001

Hygromycin B

from Streptomyces hygroscopicus

• Synonym(s): Hygromycin B from Streptomyces hygroscopicus
• Empirical Formula (Hill Notation): C₂₀H₃₇N₃O₁₃
• CAS Number: 31282-04-9
• Molecular Weight: 527.52
• UNSPSC Code: 12352200
• PubChem Substance ID: 329749191
• MDL number: MFCD06795479
• NACRES: NA.54

Properties

• biological source: Streptomyces hygroscopicus
• sterility: non-sterile; 0.2 μm filtered
• assay: 80% (HPLC)
• form: buffered aqueous solution
• composition: Hygromycin B, >80% HPLC
• packaging: pkg of 20 mL (1 g)
• manufacturer/tradename: Roche
• concentration: 50 mg/mL
• technique(s): transfection: suitable
• SMILES string: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChI key: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Description

General description

• Formula: C₂₀H₃₇N₃O₁₃
• LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
• Molecular weight: Mr = 527.5
• Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.

Application

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).[1][2][3]

Biochem/physiol Actions

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Physical form

Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane

Preparation Note

Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.

Store at 2 to 8 °C. (solution)

Analysis Note

Purity: >80% (HPLC)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.

Unit Assay: Unit/mg
We do not have this information.

Safety Information

• pictograms: GHS05,GHS06,GHS08
• signalword: Danger
• hcodes: H301,H312,H317,H318,H330,H334
• pcodes: P260 - P280 - P301 + P310 + P330 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P342 + P311 - P403 + P233
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash