10904988001

m⁷G(5′)ppp(5′)G

P¹-5′-(7-Methyl)-guanosine-P³-5′-guanosine triphosphate

• Synonym(s): methyl-guanosine-p3-5′-guanosine-triphosphate, p1-5′-7-, p¹-5′-(7-methyl)-guanosine-p³-5′-guanosine-triphosphate | methyl-guanosine-p3-5′-guanosine-triphosphate, p1-5′-7-

Properties

• Quality Level: 100
• form: lyophilized
• mol wt: 815.3
• packaging: pkg of 5 U
• manufacturer/tradename: Roche
• storage temp.: −20°C

Description

Packaging

5 units

Application

The nucleotide is used in combination with the SP6 and T7 RNA polymerase promoter system as an initiator for in vitro RNA synthesis.

Quality

Typical analysis: 90% m⁷G(5′)ppp(5′)G (from P_org.), 1.7% lithium

Specifications

Formula: C₂₁H₂₈N₁₀O₁₈P₃Li₂

Unit Definition

Conversion Factor
The data for converting A₂₅₀ units into mg is: 5 A₂₅₀ units = 0.2347 μM = 0.1914 mg.

Physical form

Lyophilizate

Preparation Note

Working concentration: The recommended working concentration with T7, T3 or SP6 RNA Polymerases is 1 mM.

Reconstitution

For best results, dissolve in water or TE at 10 mM.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable