Roche

Primer "random"

Name: Primer "random"
Brand: ROCHE
Price: 264 USD
Availability: InStock

lyophilized, sufficient for ≤400 reactions, pkg of 2 mg (Primer, Random pd(N)6 Potassium Salt)

Synonym(s):

primer

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2 MG

$264.00

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About This Item

UNSPSC Code:

41106300

NACRES:

NA.54

Key Documents

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form

lyophilized

Quality Level

100

usage

sufficient for ≤400 reactions

packaging

pkg of 2 mg (Primer, Random pd(N)6 Potassium Salt)

manufacturer/tradename

Roche

storage temp.

−20°C

Related Categories

Labware Partners

Nucleic Acid Electrophoresis & Hybridization

Nucleic Acid Labeling

Roche Life Science Products

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This Item	SML1229	70005-M	D134406
Roche 11034731001 Primer "random"	Sigma-Aldrich SML1229 2′,3′-cGAMP sodium salt	Sigma-Aldrich 70005-M T7SelectUP Primer	Sigma-Aldrich D134406 3′,5′-Dimethoxy-4′-hydroxyacetophenone
manufacturer/tradename Roche	manufacturer/tradename -	manufacturer/tradename Novagen^®	manufacturer/tradename -
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
form lyophilized	form powder	form liquid	form solid
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. -
packaging pkg of 2 mg (Primer, Random pd(N)6 Potassium Salt)	packaging -	packaging -	packaging -
usage sufficient for ≤400 reactions	usage -	usage -	usage -

General description

p(dN)₆

Application

Primer "random" has been used:

in a reverse transcription-polymerase chain reaction (RT-PCR) and quantitative PCR (qPCR) to retro-transcribe RNA[1][2]
to reverse-transcribe RNA samples to cDNA[3]
in digital droplet PCR (ddPCR) to synthesis cDNA from RNA[4]

Primer for cDNA synthesis[5][6][7]

Features and Benefits

Offers a consistent representation of all RNA sequences in mRNA
Can prime cDNA transcription from RNAs lacking a poly(A) tail
Short cDNA transcripts can overcome the challenges posed by RNA secondary structures
The average length of cDNAs formed can be controlled by adjusting the ratio of random primers to RNA in the reverse transcription (RT) reaction

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

does not flash

flash_point_c

does not flash

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Duchenne muscular dystrophy is a lethal disease caused by lack of dystrophin. Skipping of exons adjacent to out-of-frame deletions has proven to restore dystrophin expression in Duchenne patients. Exon 51 has been the most studied target in both preclinical and

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Combined treatment with the histone deacetylase inhibitor LBH589 and a splice-switch antisense oligonucleotide enhances SMN2 splicing and SMN expression in Spinal Muscular Atrophy cells.

Vittoria Pagliarini et al.

Journal of neurochemistry, 153(2), 264-275 (2019-12-08)

Spinal muscular atrophy (SMA) is a motor neuron disease caused by loss of function mutations in the Survival Motor Neuron 1 (SMN1) gene and reduced expression of the SMN protein, leading to spinal motor neuron death, muscle weakness and atrophy.

Androgen Receptor signaling promotes the neural progenitor cell pool in the developing cortex.

Piergiorgio La Rosa et al.

Journal of neurochemistry, 157(4), 1153-1166 (2020-09-23)

Neural Progenitor Cells (NPCs) are multipotent cells that are able to self-renew and differentiate into neurons. The size of the initial pool of NPCs during the brain development strongly affects the number of neurons that compose cortical multi-layer during development.

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Primer "random"