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11040596001

Roche

Interferon-γ, human (hIFN-γ)

from human, 95% (SDS-PAGE), solution, >2 x 10^7 U/mg

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Synonym(s):
SYBR, qPCR

biological source

human

Quality Level

recombinant

expressed in E. coli

description

recombinant (E. coli)

Assay

95% (SDS-PAGE)

form

solution

potency

<0.05 ng/mL EC50

specific activity

>2 x 10^7 U/mg

mol wt

16,700 Da

packaging

pkg of 100,000 U (5 μg, 1 ml)

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

impurities

<0.1 EU/μgtested (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IFNG(3458)

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This Item
SRP3058I3265IF002
Sigma-Aldrich

Sigma-Aldrich

SRP3058

IFN-γ human

Interferon-&#947; human recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), lyophilized powder, suitable for cell culture

Sigma-Aldrich

I3265

Interferon-γ human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

assay

95% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

>98% (SDS-PAGE and HPLC)

form

solution

form

lyophilized

form

lyophilized powder

form

-

potency

<0.05 ng/mL EC50

potency

5.0-10.0 ng/mL ED50

potency

-

potency

-

mol wt

16,700 Da

mol wt

16.7 kDa

mol wt

~17 kDa

mol wt

-

General description

IFN-γ is produced by T-lymphocytes stimulated by antigen or by T-cell mitogens. Under nondenaturating conditions its molecular weight values range from 32,000 to 73,000 indicating that recombinant human IFN-γ exists as a dimer or higher oligomers.

Specificity

Human IFN-γ is effective on human cells, but not on mouse or rat cells.

Application

Interferon-γ, human (hIFN-γ) has been used to stimulate mesenchymal stem cells (MSCs). It has also been used as a supplement Dulbecco′s modified Eagle′s medium/Ham′s medium for the cultivation of human colorectal carcinoma cell line T84 cells.
Interferon-γ, human (hIFN-γ), can be used to investigate IFN-γ activities in human cell systems.

Biochem/physiol Actions

A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions, antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γ′s primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells.
Interferon-γ (IFN-γ) is an essential component of acquired immunity. A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions, antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γ′s primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells.

Quality

The raw material used for this preparation was tested for HBs antigen and for the presence of antibodies to HIV-1, HIV-2, and HCV found to be negative.
Endotoxin level: <0.1EU/μg (LAL-test), <10EU/ml (LAL-test)
Note: 1EU corresponds to 0.1ng

Sequence

Chain Length 143 AA
The primary structure of recombinant human IFN-γ (143 amino acids) is identical to that of natural human IFN-γ (146 amino acids), however, recombinant IFN-γ has three amino acids less and is not glycosylated. Glycosylation is not essential for biological activity.

Unit Definition

EC50 definition: The amount of hIFN-γ that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN-γ reference standard (Gg 23– 901-530) (WISH cells-EMC virus/cytopathic effect) (1 unit equals ≤0.05 ng/ml).

Physical form

100,000U/ml or 1,000,000U/ml of Human IFN-γ in 0.1M phosphate buffer saline (PBS) (pH 7.0), 2.5% sucrose (w/v), and 2.5% human serum albumin (HSA) (w/v), filtered through a 0.2μm pore size membrane.

Preparation Note

Working solution: Solvent is recommended in double-distilled water.
Storage conditions (working solution): Stability in culture: Activity is present for at least 18 hours in culture medium.

Other Notes

EC 50 : <0.05ng/ml (hIFN-γ , NIH, reference standard, Gg 23–901-530)
For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available


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Anti-proliferative effect of IFN-gamma in immune regulation. II. IFN-gamma inhibits the proliferation of murine bone marrow cells stimulated with IL-3, IL-4, or granulocyte-macrophage colony-stimulating factor
Gajewski TF, et al.
Journal of immunology (Baltimore, Md. : 1950), 141, 2635-2642 (2019)
Drivers of heterogeneity in synovial fibroblasts in rheumatoid arthritis.
Smith, et al.
Nature Immunology, 24, 1200-1210 (2023)
Interferon-
Ellis TN, et al.
Immunology, 112, 2-2 (2004)
Simone Giovannozzi et al.
Frontiers in immunology, 11, 1114-1114 (2020-06-26)
Signal transducer and activator of transcription 1 (STAT1) gain-of-function (GOF) mutations result in a primary immunodeficiency (PID) characterized typically by chronic mucocutaneous candidiasis (CMC), but a wider phenotypic range is reported and remains unexplained from a pathophysiological point-of-view. We hypothesized
Permeability analyses and three-dimensional imaging of interferon gamma-induced barrier disintegration in intestinal organoids
Bardenbacher M, et al.
Stem Cell Research, 35, 101383-101383 (2019)

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