11051440001

dATP

=98%, solution, 100 mM, suitable for DNA sequencing, suitable for DNA amplification

• Synonym(s): 2′-Deoxyadenosine 5′-triphosphate sodium salt solution, 2′-deoxy-adenosine-5′-triphosphate, deoxy-adenosine-5′-triphosphate, 2′-, dATP
• UNSPSC Code: 41116100
• NACRES: NA.54
• Assay: 98%
• Form: solution
• Storage temp.: −20°C

Properties

• InChI: 1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1
• InChI key: YJWCICGGRLOGEH-VWZUFWLJSA-M
• SMILES string: [Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3
• description: Lithium salt
• assay: 98%
• form: solution
• usage: sufficient for 6,250 standard reactions (PCR)
• packaging: pkg of 250 μL (25 μmol; 100 mM)
• manufacturer/tradename: Roche
• concentration: 100 mM
• technique(s): DNA amplification: suitable, DNA sequencing: suitable, DNA synthesis: suitable, PCR: suitable
• storage temp.: −20°C
• Quality Level: 100

Description

General description

2′-Deoxyadenosine 5′-triphosphate (dATP) is made up of a nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar bound to a chain of three phosphate residues. dATP is used by cells for synthesis of DNA by DNA polymerases.

dATP is a 100 mM clear, colorless solution of the lithium salt (pH 7).Download the CofA, SDS and IFU s

Application

2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.

Use dATP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

dATP has been used to resume transcription.[1]

Biochem/physiol Actions

Deoxyadenosine-5-triphosphate (dATP) plays a major role in the initiation of apoptosis in vitro.[2] dATP is considered as an inhibitor of mammalian ribonucleotide reductase, thereby DNA synthesis.[3]

Analysis Note

Typical analysis:

• ≤98% dATP (HPLC), ≤1.5% dADP (HPLC), ≤0.5% dAMP (HPLC)
• Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR

Other Notes

1 μmol dATP = 15.0 A₂₆₀ units

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:

• nucleotide sets (all 4 nucleotides in separate vials)
• ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:

• kits (dNTPs, buffer, and polymerase in separate vials)
• ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: No data available
• flash_point_c: No data available