11051466001

dGTP

=98%, solution, 100 mM, suitable for PCR, suitable for DNA sequencing

• Synonym(s): 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, 2′-deoxy-guanosine-5′-triphosphate, deoxy-guanosine-5′-triphosphate, 2′-, dGTP-Na₃
• Empirical Formula (Hill Notation): C₁₀H₁₃N₅Na₃O₁₃P₃
• CAS Number: 93919-41-6
• Molecular Weight: 573.13
• MDL number: MFCD00080337
• PubChem Substance ID: 329749301

Properties

• Quality Level: 100
• description: Lithium salt
• Assay: 98%
• form: solution
• usage: sufficient for 6,250 standard reactions (PCR)
• packaging: pkg of 250 μL (25 μmol; 100 mM)
• manufacturer/tradename: Roche
• concentration: 100 mM
• technique(s): DNA amplification: suitable; DNA sequencing: suitable; DNA synthesis: suitable; PCR: suitable
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1
• InChI: 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
• InChI key: IWGGLKOTEOCWQP-BIHLCPNHSA-K

Description

General description

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

dGTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dGTP solution in water (pH 7), 100 mM, lithium salt.

Application

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

Use dGTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

Quality

Typical analysis:

• ≥98% dGTP (HPLC), ≤1.5% dGDP (HPLC), ≤0.5% dGMP (HPLC)
• Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR

Unit Definition

1 μmol dGTP = 13.7 A₂₅₂ units

Other Notes

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:

• nucleotide sets (all 4 nucleotides in separate vials)
• ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:

• kits (dNTPs, buffer, and polymerase in separate vials)
• ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): No data available
• Flash Point(C): No data available