11088947001

BM Condimed H1

Hybridoma Cloning Supplement

• Synonym(s): hybridoma cloning supplement
• UNSPSC Code: 12352207
• NACRES: NA.54

Properties

• sterility: 0.2 μm filtered
• form: solution
• packaging: pkg of 100 mL (10x)
• manufacturer/tradename: Roche
• technique(s): cell culture | hybridoma: suitable
• solubility: water: miscible
• storage temp.: −20°C

Description

General description

BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.

Application

BM Condimed H1 is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning.[1][2] BM Condimed H1 replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.

Biochem/physiol Actions

BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.

Features and Benefits

Contents
Solution in RPMI 1640, filtered through 0.2μm pore size membrane

Physical form

Solution in RPMI 1640, filtered through 0.2 μm pore size membrane

Preparation Note

Working concentration: 10% (v/v) in culture medium that contains 10 - 20% FBS.

Analysis Note

Biological activity: Assayed for its ability to promote the growth of freshly fused hybridoma cells.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash