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Blocking Reagent

For nucleic acid hybridization and detection

blocking reagent

Quality Level




pkg of 50 g



shipped in


storage temp.



The Blocking Reagent is used to decrease the background in nonradioactive filter hybridization and the detection of nucleic acid hybrids. It has also been used as a blocking agent in immunohistochemistry.

Preparation Note

Working concentration: Dilution of Antibody 1:5,000
Working solution: To prepare a 10x blocking stock solution Blocking Reagent is dissolved in maleic acid buffer to a final concentration of 10% (w/v) with shaking and heating either on a heating block or in a microwave oven.
Storage conditions (working solution): 10x stock solution of Blocking Reagent is autoclaved and stored at 2 to 8 °C. Alternativly, aliquots can be stored at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Quotes and Ordering

Nuria de Pedro et al.
Biological procedures online, 22, 2-2 (2020-01-21)
A large number of studies have suggested a correlation between the status of telomeres and disease risk. High-throughput quantitative fluorescence in situ hybridization (HT Q-FISH) is a highly accurate telomere measurement technique that can be applied to the study of
Richard C Krolewski et al.
Scientific reports, 10(1), 1781-1781 (2020-02-06)
The family of olfactory receptors (ORs) subserves the sense of smell and includes both functional alleles and pseudogenes, the latter identified by mutations resulting in frame shift or premature truncation. During neuronal differentiation, nonfunctional ORs are expressed initially but then
Nodal promotes functional luteolysis via down-regulation of progesterone and prostaglandins E2 and promotion of PGF2α synthetic pathways in mare corpus luteum.
Galv?o A, et al.
Endocrinology, 157(2), 858-871 (2015)
Revised lineage of larval photoreceptor cells in Ciona reveals archetypal collaboration between neural tube and neural crest in sensory organ formation.
Oonuma K, et al.
Developmental Biology, 420(1), 178-185 (2016)
Christina-Marie Baumbach et al.
Mycoses, 63(9), 970-978 (2020-07-04)
Dermatophytoses rank among the most frequent communicable diseases in humans, and the zoonotic transmission is increasing. The zoophilic dermatophyte Trichophyton (T.) benhamiae is nowadays one of the main causes of tinea faciei et corporis in children. However, scientific data on


Blocking Reagent Protocol & Troubleshooting

The easiest way to use the Blocking Reagent is with the Roche DIG Wash and Block Buffer Set, a ready-to-use set of stock solutions.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service