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11096176001

Roche

Blocking Reagent

For nucleic acid hybridization and detection

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Synonym(s):
blocking reagent

Quality Level

100

form

powder

packaging

pkg of 50 g

manufacturer/tradename

Roche

storage temp.

20-25°C

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This Item
WBAVDCH01W0138G7663
Blocking Reagent For nucleic acid hybridization and detection

Roche

11096176001

Blocking Reagent

Millipore

Millipore

WBAVDCH01

Immobilon® Block - CH (Chemiluminescent Blocker)

Sigma-Aldrich

Sigma-Aldrich

W0138

Western Blocker™ Solution

Sigma-Aldrich

Sigma-Aldrich

G7663

Gelatin blocking buffer

form

powder

form

-

form

-

form

powder blend

manufacturer/tradename

Roche

manufacturer/tradename

Immobilon®

manufacturer/tradename

-

manufacturer/tradename

-

storage temp.

20-25°C

storage temp.

15-25°C

storage temp.

2-8°C

storage temp.

room temp

Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

-

Application

The Blocking Reagent is used to decrease the background in nonradioactive filter hybridization and the detection of nucleic acid hybrids. It has also been used as a blocking agent in immunohistochemistry.

Preparation Note

Working concentration: Dilution of Antibody 1:5,000
Working solution: To prepare a 10x blocking stock solution Blocking Reagent is dissolved in maleic acid buffer to a final concentration of 10% (w/v) with shaking and heating either on a heating block or in a microwave oven.
Storage conditions (working solution): 10x stock solution of Blocking Reagent is autoclaved and stored at 2 to 8 °C. Alternativly, aliquots can be stored at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Protocols

Blocking Reagent Protocol & Troubleshooting

The easiest way to use the Blocking Reagent is with the Roche DIG Wash and Block Buffer Set, a ready-to-use set of stock solutions.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service