MilliporeSigma
All Photos(1)

11117831001

Roche

cDNA Synthesis Kit

kit of for up to 10 reactions

Synonym(s):
RT-PCR

Quality Level

manufacturer/tradename

Roche

packaging

kit of for up to 10 reactions

General description

Optimized one-tube procedure for the synthesis of double-stranded cDNA up to 3 kb from total RNA or mRNA.
The cDNA Synthesis System uses an optimized one-tube procedure, based on the method of Gubler and Hoffmann.
Sample: 1 - 20 μg total RNA, 0.3 - 2 μg mRNA
Assay time: 60 minutes for 1st strand synthesis, 120 minutes for 2nd strand synthesis
Product: double-stranded cDNA copy of an RNA sample

Application

Optimized one-tube procedure for the synthesis of double-stranded cDNA from total RNA or mRNA.
The ds cDNA can be used for the construction of non-directional cDNA libraries, as a starting point for substractive hybridization experiments to enrich differentially expressed genes, and for the in vitro transcription of whole cDNA populations to generate labeled cRNA for hybridization on DNA microarrays.

Components

  • AMV Reverse Transcriptase Buffer, 5x concentrated
  • AMV Reverse Transcriptase (25 U/μl)
  • 1,4-Dithiothreithol (0.1 M)
  • Protector RNase Inhibitor (25 U/μl)
  • Oligo (dT)15 primer, 200 μM (1 μg/μl)
  • Oligo [(dT24 )T7promotor]65 primer, 100 μM (2 μg/μl)
  • dNTP mixture (each nucleotide, 10 mM)
  • Control neo mRNA (0.2 μg/μl)
  • 2nd strand synthesis buffer, 5x concentrated
  • 2nd strand enzyme blend (mixture of DNA Polymerase I, E. coli Ligase and RNase H)
  • T4 DNA Polymerase (1 U/μl)
  • Double-distilled water
  • RNase I (10 U/μl)
  • Proteinase K, recombinant, PCR grade (50 U/mll)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Aakansha Sharma et al.
Experimental physiology, 103(4), 559-569 (2018-01-31)
What is the central question of this study? What are the molecular underpinnings of the seasonal adaptation in a latitudinal migratory songbird? What is the main finding and its importance? We found changes in mRNA levels after a photoperiod-induced alteration
Weidong Liu et al.
Experimental brain research, 239(11), 3397-3404 (2021-09-10)
Our objective of this study is to determine the molecular mechanism of MAPKs (mitogen activated protein kinase systems) on TRPV4 (transient receptor potential vanilloid 4)-mediated trigeminal neuralgia (TN). Partial chronic constriction injury of the infraorbital nerve (CCI-ION) ligation model was
Reeja Maria Cherian et al.
Glycobiology, 24(1), 26-38 (2013-10-02)
The binding of Shiga-like toxin 1 (Stx1) and Shiga-like toxin 2 (Stx2) to a mucin-like fusion protein, P-selectin glycoprotein ligand-1/mouse IgG2b (PSGL-1/mIgG2b), carrying multiple copies of the blood group P1 determinant on O-glycans was investigated with western blot and the
Zhe Quan et al.
Technology in cancer research & treatment, 18, 1533033819892258-1533033819892258 (2019-12-25)
Basal cell carcinoma is driven by the aberrant activation of hedgehog signaling. DEAD (Asp-Glu-Ala-Asp) box protein 5 is frequently overexpressed in human cancer cells and associated with the tumor growth and invasion. The purpose of this study was to investigate
Dorota Sołtys-Kalina et al.
Molecular breeding : new strategies in plant improvement, 35(12), 224-224 (2015-11-28)
Potato (Solanum tuberosum L.) tubers exhibit significant variation in reducing sugar content directly after harvest, cold storage and reconditioning. Here, we performed QTL analysis for chip color, which is strongly influenced by reducing sugar content, in a diploid potato mapping

Related Content

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service