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11117831001

Roche

cDNA Synthesis Kit

kit of for up to 10 reactions

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Synonym(s):
RT-PCR

manufacturer/tradename

Roche

packaging

kit of for up to 10 reactions

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This Item
05893151001GE27-9261-01THIFICDNARO
cDNA Synthesis Kit kit of for up to 10 reactions

Roche

11117831001

cDNA Synthesis Kit

Transcriptor Universal cDNA Master sufficient for 100 reactions, suitable for cDNA synthesis, suitable for RT-PCR, suitable for RT-qPCR

Roche

05893151001

Transcriptor Universal cDNA Master

First-strand cDNA Synthesis Kit Cytiva 27-9261-01

GE27-9261-01

First-strand cDNA Synthesis Kit

Transcriptor High Fidelity cDNA Synthesis Kit sufficient for 50 reactions, sufficient for 100 reactions, sufficient for 200 reactions, suitable for RT-PCR, suitable for RT-qPCR

Roche

THIFICDNARO

Transcriptor High Fidelity cDNA Synthesis Kit

packaging

kit of for up to 10 reactions

packaging

kit of for 100 reactions

packaging

-

packaging

pkg of 100 reactions (05091284001), pkg of 200 reactions (05081963001), pkg of 50 reactions (05081955001)

General description

Optimized one-tube procedure for the synthesis of double-stranded cDNA up to 3 kb from total RNA or mRNA.
The cDNA Synthesis System uses an optimized one-tube procedure, based on the method of Gubler and Hoffmann.
Sample: 1 - 20 μg total RNA, 0.3 - 2 μg mRNA
Assay time: 60 minutes for 1st strand synthesis, 120 minutes for 2nd strand synthesis
Product: double-stranded cDNA copy of an RNA sample

Application

Optimized one-tube procedure for the synthesis of double-stranded cDNA from total RNA or mRNA.
The ds cDNA can be used for the construction of non-directional cDNA libraries, as a starting point for substractive hybridization experiments to enrich differentially expressed genes, and for the in vitro transcription of whole cDNA populations to generate labeled cRNA for hybridization on DNA microarrays.

Components

  • AMV Reverse Transcriptase Buffer, 5x concentrated
  • AMV Reverse Transcriptase (25 U/μl)
  • 1,4-Dithiothreithol (0.1 M)
  • Protector RNase Inhibitor (25 U/μl)
  • Oligo (dT)15 primer, 200 μM (1 μg/μl)
  • Oligo [(dT24 )T7promotor]65 primer, 100 μM (2 μg/μl)
  • dNTP mixture (each nucleotide, 10 mM)
  • Control neo mRNA (0.2 μg/μl)
  • 2nd strand synthesis buffer, 5x concentrated
  • 2nd strand enzyme blend (mixture of DNA Polymerase I, E. coli Ligase and RNase H)
  • T4 DNA Polymerase (1 U/μl)
  • Double-distilled water
  • RNase I (10 U/μl)
  • Proteinase K, recombinant, PCR grade (50 U/mll)

Other Notes

Reverse Transcriptases Overview
For life science research only. Not for use in diagnostic procedures.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H319 - H334

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(C)

does not flash

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RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.

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