11175041910
Roche
DIG Nucleic Acid Detection Kit
sufficient for 40 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization, suitable for Northern blotting
Synonym(s):
DIG system
About This Item
Recommended Products
usage
sufficient for 40 blots (10 cm x 10 cm each)
Quality Level
packaging
kit of 1 (5 components)
manufacturer/tradename
Roche
greener alternative product characteristics
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sustainability
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technique(s)
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
greener alternative category
, Aligned
storage temp.
−20°C
General description
Application
- Southern blots
- Northern blots
- Other nucleic acid blotting applications
- In situ hybridization applications
- Southern blots
- Northern blots
- Other nucleic acid blotting applications
- In situ hybridization applications
Features and Benefits
Packaging
Principle
Preparation Note
Working concentration of conjugate depends on application and substrate.
Storage conditions (working solution):
- Anti-Digoxigenin-AP Conjugate (vial 3): 2 to 8 °C, it is stable for 12 months at this temperature
- NBT/BCIP (vial 4): 2 to 8 °C, stable
Note: During shipment of the kit on dry ice, a precipitate may occur which is dissolved by briefly warming to 37 °C - The blocking reagent (vial 5) is stable for 36 months and can be stored dry at 4 to 8 °C.
- Autoclaved stock solution can be stored for several days to a week either unopened at 15 to 25 °C or at 4 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.
Kit Components Only
- DIG-labeled Control DNA 5 µg/ml
- DNA Dilution Buffer, [50 μg/ml] fish sperm DNA
- Anti-Digoxigenin-AP Conjugate antibody 750 U/ml
- NBT/BCIP, 50x stock solution 50x concentrated
- Blocking Reagent
wgk_germany
WGK 3
flash_point_f
does not flashNot applicable
flash_point_c
does not flashNot applicable
Certificates of Analysis (COA)
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Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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