11175041910

DIG Nucleic Acid Detection Kit

sufficient for 40 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization, suitable for Northern blotting

• Synonym(s): DIG system
• UNSPSC Code: 41116134
• NACRES: NA.54

Properties

• usage: sufficient for 40 blots (10 cm x 10 cm each)
• packaging: kit of 1 (5 components)
• manufacturer/tradename: Roche
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): Northern blotting: suitable, Southern blotting: suitable, hybridization: suitable
• greener alternative category: , Aligned
• storage temp.: −20°C

Description

General description

DIG Nucleic Acid Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG nucleic acid detection kit has been used in the confirmation of genetic manipulation.

The DIG Nucleic Acid Detection Kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:

• Southern blots
• Northern blots[1]
• Other nucleic acid blotting applications
• In situ hybridization applications[2]

The kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:

• Southern blots
• Northern blots
• Other nucleic acid blotting applications
• In situ hybridization applications

Biochem/physiol Actions

DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP antibody conjugate and the color substrates NBT and BCIP.

Features and Benefits

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Packaging

1 kit containing 5 components.

Preparation Note

Working concentration: Flow cytometry: 2 μg/ml (0.2 μg/100 μl/106 cells); Immunohistocytochemistry: 6 μg/ml
Working concentration of conjugate depends on application and substrate.
Storage conditions (working solution):

• Anti-Digoxigenin-AP Conjugate (vial 3): 2 to 8 °C, it is stable for 12 months at this temperature
• NBT/BCIP (vial 4): 2 to 8 °C, stable
  Note: During shipment of the kit on dry ice, a precipitate may occur which is dissolved by briefly warming to 37 °C
• The blocking reagent (vial 5) is stable for 36 months and can be stored dry at 4 to 8 °C.
• Autoclaved stock solution can be stored for several days to a week either unopened at 15 to 25 °C or at 4 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.

Other Notes

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Kit Components Only

• DIG-labeled Control DNA 5 µg/ml
• DNA Dilution Buffer, [50 μg/ml] fish sperm DNA
• Anti-Digoxigenin-AP Conjugate antibody 750 U/ml
• NBT/BCIP, 50x stock solution 50x concentrated
• Blocking Reagent

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: does not flashNot applicable
• flash_point_c: does not flashNot applicable