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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 125 mL | Check Cart for Availability | $68.80 |
About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
$68.80
Check Cart for Availability
form
solution
packaging
pkg of 125 mL
manufacturer/tradename
Roche
shipped in
wet ice
storage temp.
2-8°C
General description
2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.[1]
Application
Preparation Note
Working concentration: Flow cytometry: 0.5 μg/100 μl (106 cells); Immunohistocytochemistry: 50 μg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ABTS is a trademark of Roche
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This Item | |||
|---|---|---|---|
| form solution | form solution (ready-to-use) | form liquid | form solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. room temp |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Chemicon® | manufacturer/tradename - |
| packaging pkg of 125 mL | packaging pkg of 3 × 100 mL | packaging - | packaging - |
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nwg
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No data available
flash_point_c
No data available
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 11204530001 | 04061838250711 |