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11207750910

Roche

Anti-Digoxigenin-Rhodamine, Fab fragments

from sheep

Synonym(s):

anti-digoxigenin, digoxigenin

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About This Item

UNSPSC Code:
12352203

biological source

sheep

Quality Level

conjugate

rhodamine conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized (clear, red solution after reconstitution)

packaging

pkg of 200 μg

manufacturer/tradename

Roche

isotype

IgG

storage temp.

2-8°C

General description

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to rhodamine.

Specificity

Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).

Application

Use Anti-Digoxigenin-Rhodamine, Fab fragments for the detection of digoxigenin-labeled compounds using:
  • Digoxigenin-labeled sugars in glycoconjugate research
  • Fluorescent in situ hybridization (FISH)
  • Immunohistocytochemistry
  • In situ hybridization
  • DNA fiber assay

Preparation Note

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Detection of digoxigenin-labeled sugars in glycoconjugates: 20 to 50 μg/ml
  • Fluorescent in situ hybridization (FISH): 1 to 20 μg/ml
  • Immunohistocytochemistry: 20 to 50 μg/ml
  • In situ hybridization: 20 to 50 μg/ml

Working solution: PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore pH can be increased up to pH 8.5 to 9.0.

Storage conditions (working solution): Always prepare fresh!

Reconstitution

Add 1 ml double-distilled water to a final concentration of 200 μg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Camila A Quercia et al.
Comparative cytogenetics, 14(1), 61-74 (2020-02-12)
South American frogs of the genus Eupsophus Fitzinger, 1843 comprise 10 species. Two of them, Eupsophus vertebralis Grandison, 1961 and E. emiliopugini Formas, 1989 belong to the Eupsophus vertebralis group, exhibiting 2n = 28. Fundamental number differences between these species
Galina Pendinen et al.
Genome, 51(9), 714-720 (2008-09-06)
Thirty-six percent of the wild potato (Solanum L. section Petota Dumort.) species are polyploid, and about half of the polyploids are tetraploid species (2n = 4x = 48). Determination of the type of polyploidy and development of the genome concept
R Symonová et al.
Cytogenetic and genome research, 141(2-3), 153-162 (2013-09-21)
We applied comparative genomic hybridization (CGH) and genomic in situ hybridization (GISH) to examine genomes of artificially produced sturgeon hybrids between sterlet, Acipenser ruthenus female (∼120 chromosomes) or Russian sturgeon, A. gueldenstaedtii female (∼240 chromosomes) and a spontaneous triploid Siberian
Shin-Ichiro Takebayashi et al.
Epigenetics & chromatin, 6(1), 42-42 (2013-12-18)
Cellular differentiation and reprogramming are accompanied by changes in replication timing and 3D organization of large-scale (400 to 800 Kb) chromosomal domains ('replication domains'), but few gene products have been identified whose disruption affects these properties. Here we show that
Maximilian H Fitz-James et al.
eLife, 9 (2020-09-12)
During mitosis chromosomes reorganise into highly compact, rod-shaped forms, thought to consist of consecutive chromatin loops around a central protein scaffold. Condensin complexes are involved in chromatin compaction, but the contribution of other chromatin proteins, DNA sequence and histone modifications

Articles

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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