11209264001

DNA Molecular Weight Marker VII

pkg of 50 μg (in 200 μl), solution

• Synonym(s): DNA marker
• UNSPSC Code: 41105335
• NACRES: NA.54
• eCl@ss: 32119000
• Form: solution
• Solubility: water: miscible
• Storage temp.: −20°C

Properties

• form: solution
• packaging: pkg of 50 μg (in 200 μl)
• manufacturer/tradename: Roche
• concentration: 250 μg/mL
• color: colorless
• solubility: water: miscible
• storage temp.: −20°C

Description

General description

Contents
Ready-to-use solution, 250 μg/ml, in TE buffer (Tris-HCl, 1 mM EDTA, pH 8.0).

Fragment mixture prepared by cleavage of SPP1-DNA with restriction endonuclease EcoR I.
The product is also available as DNA Molecular Weight Marker VII, Digoxigenin-labeled.
Size Range: 0.081 to 8.57 kbp

Application

DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

• Restriction digests
• PCR
• RT-PCR

Preparation Note

Storage conditions (working solution): 2 to 8 °C
Once thawed store at 2 to 8 °C.

Other Notes

50 μg = 1A₂₆₀ unit

For life science research only. Not for use in diagnostic procedures.

The mixture contains seventeen fragments with the following base pair lengths (1 base pair = 660 daltons): 81, 359, 492, 710, 718*, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899, 6106, 7427, and 8576 as determined by computer analysis of the SSP1 sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
*Alonso, J.C. and Trautner, T.A., personal communication of unpublished results, the 718-bp fragment is present in non-stoichometric amounts.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash