11243217001

Laminin

from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

• Synonym(s): Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
• CAS Number: 114956-81-9
• MDL number: MFCD00081739
• UNSPSC Code: 12352207
• NACRES: NA.54

Properties

• biological source: mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)
• Quality Level: 100
• sterility: sterile; sterile-filtered
• assay: ≥90% (SDS-PAGE)
• form: solution
• mol wt: 900 kDa
• packaging: pkg of 1 mg (2 ml)
• manufacturer/tradename: Roche
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: Q61292
• storage temp.: −20°C
• Gene Information: mouse ... Lamb2(16779)

Description

General description

Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes.[1] Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.

Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.

Application

Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes.[2][3] It has been used for cell adhesion assay.[4] It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm². The optimal concentration does depend on cell type as well as the application or research objectives.

Physical form

Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile

Solution, filtered through 0.2 ?m pore size membrane

Preparation Note

Working concentration: 2 to 5 μg/cm²
2 to 5 μg/cm² for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.

Analysis Note

Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)

Other Notes

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Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: does not flash
• flash_point_c: does not flash