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| Size/SKU | Availability | Price |
|---|---|---|
5 mg | Available to ship TODAYfromMILWAUKEE | $451.00 |
About This Item
UNSPSC Code:
23201100
NACRES:
NA.54
Form:
lyophilized (clear, colorless solution after reconstitution)
Assay:
>95% (SDS-PAGE)
$451.00
Available to ship TODAYDetails
sterility
non-sterile; 0.2 μm filtered
assay
>95% (SDS-PAGE)
form
lyophilized (clear, colorless solution after reconstitution)
packaging
pkg of 5 mg
manufacturer/tradename
Roche
application(s)
hematology
storage temp.
2-8°C
General description
PHA-L is purified from Phaseolus vulgaris (red kidney bean) by standard chromatographic techniques.
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean, consists of only L-type subunits (isolectin L4, "leuko-agglutinin"). It is an N-acetylgalactosamine/galactose sugar-specific lectin. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.[1]
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean, consists of only L-type subunits (isolectin L4, "leuko-agglutinin"). It is an N-acetylgalactosamine/galactose sugar-specific lectin. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.[1]
Application
Phytohemagglutinin-L (PHA-L) consists of only L-type subunits (isolectin L4, "leuko-agglutinin"), and is well suited for high-efficiency induction and the functional analysis of T-lymphocytes.[2] It is highly purified and tested for high-efficiency stimulation of human lymphocytes. It is also used as positive controls for peripheral blood mononuclear cells (PBMC) stimulation.
Biochem/physiol Actions
Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.[1]
Features and Benefits
Biological activity: <5μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes
Preparation Note
Working concentration: 1 to 5 μg/ml
Approximately 1 to 5 μg/ml for the stimulation of human peripheral blood lymphocytes
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Approximately 1 to 5 μg/ml for the stimulation of human peripheral blood lymphocytes
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Other Notes
For life science research only. Not for use in diagnostic procedures.
PHA-L is a tetramer of 4 L-type subunits held together by noncovalent forces.
1 of 1
This Item | |||
|---|---|---|---|
| assay >95% (SDS-PAGE) | assay - | assay - | assay - |
| form lyophilized (clear, colorless solution after reconstitution) | form lyophilized powder | form lyophilized | form suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. - |
| packaging pkg of 5 mg | packaging - | packaging - | packaging pkg of 10 mL (100 mg) |
| sterility non-sterile; 0.2 μm filtered | sterility - | sterility - | sterility - |
| application(s) hematology | application(s) - | application(s) - | application(s) - |
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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