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11284932001

Roche

DNase I

from bovine pancreas

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Synonym(s):
Dnase
Enzyme Commission number:

biological source

bovine pancreas

Quality Level

form

lyophilized

packaging

pkg of 100 mg

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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This Item
D4527AMPD1D5025
DNase I from bovine pancreas

11284932001

DNase I

DNase I Amplification Grade

AMPD1

DNase I

biological source

bovine pancreas

biological source

-

biological source

-

biological source

bovine pancreas

form

lyophilized

form

lyophilized powder

form

liquid

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

300

Quality Level

-

Quality Level

-

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

General description

DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin.

Application

DNase I has been used for tissue dissociation in conjunction with collagenases.

Biochem/physiol Actions

During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.

Features and Benefits

  • Lyophilized
  • Filtered through 0.2 μm pore size membrane before lyophilization
  • Glycoprotein and double-strand-specific endonuclease

Unit Definition

One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.

Preparation Note

Activator: Bivalent metal ions (Ca2+, Mg2+)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.

Reconstitution

Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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