TWEEN^® 20

Name: TWEEN<SUP>®</SUP> 20
Brand: ROCHE
Price: 140 USD

non-ionic, aqueous solution, 10% (w/v)

Synonym(s):

TWEEN 20, Tween, Tween 20

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5 X 10 ML

$140.00

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.54

Key Documents

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description

Formula: C₅₈H₁₁₄O₂₆, for n = 20

form

solution

mol wt

M_r 1228 (for n= 20)

packaging

pkg of 5 × 10 mL

manufacturer/tradename

Roche

technique(s)

cell culture | plant: suitable, microbiological culture: suitable

CMC

~0.06 mM

absorption

= 0.025 at 254 nm at 0.05 % (w/v)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Biochemicals

Cell Culture & Analysis

Cell Culture Media & Buffers

Cell Culture Supplements & Reagents

Detergents - Anionic, Cationic, Zwitterionic, Anti-foaming

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This Item	P2287	P7949	8.22184
Sigma-Aldrich 11332465001 TWEEN^® 20	Sigma-Aldrich P2287 TWEEN^® 20	Sigma-Aldrich P7949 TWEEN^® 20	Sigma-Aldrich 8.22184 TWEEN^® 20
form solution	form viscous liquid	form viscous liquid	form liquid
technique(s) cell culture \| plant: suitable, microbiological culture: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) blocking: suitable, cell culture \| mammalian: suitable, hybridization: suitable, protein purification: suitable, transformation: suitable	technique(s) -
storage temp. 2-8°C	storage temp. -	storage temp. -	storage temp. 2-30°C
CMC ~0.06 mM	CMC 0.06 mM (20-25°C)	CMC 0.06 mM (20-25°C)	CMC -
mol wt M_r 1228 (for n= 20)	mol wt ~1228	mol wt ~1228	mol wt ~1228
description Formula: C₅₈H₁₁₄O₂₆, for n = 20	description non-ionic	description non-ionic	description -

General description

Tween 20, also referred to as poly(oxyethylene)_n-sorbitan-monolaurate, is a non-ionic surfactant. It is mainly used in membrane research.[1]

Application

Tween 20 is used for:

solubilizing membrane proteins during isolation of membrane-protein complexes.
a subsequent protein detection by UV light absorption, due to its low UV absorbance.
ELISA (enzyme-linked immunosorbent assay) and immunoblots.

Physical form

10% (w/v) aqueous solution under nitrogen, supplied in injection glass bottles.

Preparation Note

Working concentration: >10 mg/mg membrane lipid

Analysis Note

Purity: <2 ppm peroxide content (as H₂O₂), <200 μM carbonyls, <100 μS conductivity. Specially purified for membrane research.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available

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Effects of Tween 20 and Tween 80 on the stability of Albutropin during agitation.

Danny K Chou et al.

Journal of pharmaceutical sciences, 94(6), 1368-1381 (2005-04-29)

The objectives of this work were to determine the effects of nonionic surfactants (Tween 20 and Tween 80) on agitation-induced aggregation of the recombinant fusion protein, Albutropintrade mark (human growth hormone genetically fused to human albumin), and to characterize the

TWEEN® 20