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11332465001

Sigma-Aldrich

TWEEN® 20

non-ionic, aqueous solution, 10% (w/v)

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Synonym(s):
TWEEN 20, Tween, Tween 20

description

Formula: C58H114O26, for n = 20

form

solution

mol wt

Mr 1228 (for n= 20)

packaging

pkg of 5 × 10 mL

manufacturer/tradename

Roche

technique(s)

cell culture | plant: suitable
microbiological culture: suitable

CMC

~0.06 mM

absorption

= 0.025 at 254 nm at 0.05 % (w/v)

shipped in

wet ice

storage temp.

2-8°C

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TWEEN® 20 non-ionic, aqueous solution, 10% (w/v)

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TWEEN® 20 viscous liquid, suitable for cell culture

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form

solution

form

viscous liquid

form

viscous liquid

form

liquid

mol wt

Mr 1228 (for n= 20)

mol wt

~1228

mol wt

~1228

mol wt

~1228

technique(s)

cell culture | plant: suitable, microbiological culture: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

blocking: suitable, cell culture | mammalian: suitable, hybridization: suitable, protein purification: suitable, transformation: suitable

technique(s)

-

CMC

~0.06 mM

CMC

0.06 mM (20-25°C)

CMC

0.06 mM (20-25°C)

CMC

-

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

2-30°C

General description

Tween 20, also referred to as poly(oxyethylene)n-sorbitan-monolaurate, is a non-ionic surfactant. It is mainly used in membrane research.

Application

Tween 20 is used for:
  • solubilizing membrane proteins during isolation of membrane-protein complexes.
  • a subsequent protein detection by UV light absorption, due to its low UV absorbance.
  • ELISA (enzyme-linked immunosorbent assay) and immunoblots.

Quality

Purity: <2 ppm peroxide content (as H2O2), <200 μM carbonyls, <100 μS conductivity. Specially purified for membrane research.

Physical form

10% (w/v) aqueous solution under nitrogen, supplied in injection glass bottles.

Preparation Note

Working concentration: >10 mg/mg membrane lipid

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available


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Danny K Chou et al.
Journal of pharmaceutical sciences, 94(6), 1368-1381 (2005-04-29)
The objectives of this work were to determine the effects of nonionic surfactants (Tween 20 and Tween 80) on agitation-induced aggregation of the recombinant fusion protein, Albutropintrade mark (human growth hormone genetically fused to human albumin), and to characterize the

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