11333054001

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester

>90% (elementary analysis), pkg of 5 mg, powder, pH range 2-13

• Synonym(s): Digoxigenin NHS-ester, dig-nhs-ester, ε-(Digoxigenin-3-0-acetamido)caproic acid N-hydroxysuccinimide ester, DIG NHS
• Empirical Formula (Hill Notation): C₃₅H₅₀N₂O₁₀
• CAS Number: 129273-26-3
• Molecular Weight: 658.78
• UNSPSC Code: 41105600
• PubChem Substance ID: 329749387
• MDL number: MFCD02093394
• NACRES: NA.54
• Assay: >90% (elementary analysis)
• Form: powder

Properties

• Quality Level: 100
• assay: >90% (elementary analysis)
• form: powder
• mol wt: M_r 658.8
• packaging: pkg of 5 mg
• manufacturer/tradename: Roche
• pH range: 2-13
• solubility: DMF: soluble, DMSO: soluble, ethanol: soluble
• storage temp.: −20°C
• SMILES string: [H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O
• InChI: 1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1
• InChI key: KHNDABJZSPPYLE-FUGFVFQCSA-N

Description

General description

Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH₂-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

Application

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).[1]

Biochem/physiol Actions

The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.

Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.

Preparation Note

Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- ε-aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 μl DMSO or ethanol.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS06,GHS08,GHS09
• signalword: Danger
• hcodes: H300,H319,H331,H373,H410
• pcodes: P260 - P264 - P273 - P301 + P310 + P330 - P391 - P403 + P233
• Hazard Classifications: Acute Tox. 2 Oral - Acute Tox. 3 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - STOT RE 2
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable