11363735001

Hybridoma Fusion and Cloning Supplement

solution, suitable for hybridoma, Mycoplasma, tested

• Synonym(s): HFCS
• UNSPSC Code: 12352207
• NACRES: NA.54

Properties

• description: HFCS
• sterility: 0.2 μm filtered
• form: solution
• packaging: pkg of 10 mL (50x)
• manufacturer/tradename: Roche
• technique(s): cell culture | hybridoma: suitable
• impurities: Mycoplasma, tested, 10 EU/mL Endotoxin (LAL)
• solubility: water: miscible
• storage temp.: −20°C

Description

General description

Medium supplement composed of albumin, insulin, transferrin, cytokines, a cholesterol source, other defined organic and inorganic compounds and fetal calf serum (final concentration: 0.5%).
HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures).

Application

Hybridoma Fusion and Cloning Supplement (HFCS) is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. HFCS replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.[1][2]

Physical form

Solution (50x concentrated, pH 7.4); filtered through 0.2 μm pore size membrane

Preparation Note

Working concentration: HFCS (50x) is diluted 1:50 (v/v) with basal medium. It is strongly suggested that you use RPMI 1640. The final medium should also contain L-glutamine and sodium bicarbonate.

Analysis Note

Biological activity: Assayed for high cloning efficiency of a hybridoma cell line.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash