11363905910

Digoxigenin-11-ddUTP

solution, =85% (HPLC), pkg of 25 μL (25 nmol; 1mM)

• Synonym(s): digoxigenin
• UNSPSC Code: 41116100

Properties

• Quality Level: 100
• assay: 85% (HPLC)
• form: solution
• mol wt: (Molecular weight: M_r = 1074.7 (DIG-11-ddUTP-Li₄))
• packaging: pkg of 25 μL (25 nmol; 1mM)
• manufacturer/tradename: Roche
• storage temp.: −20°C

Description

General description

Digoxigenin-11-ddUTP is a 1 mM solution of the tetralithium salt. Digoxigenin-11-ddUTP is a component of probe used for end labeling.[1] Digoxigenin-11-ddUTP is an alternative to classical radioactive labeling for electrophoretic mobility shift assay and is highly stable compared to radioactive probes.[2]

Application

Digoxigenin-11-ddUTP has been used:

• for labeling of DNA fragments in linker histone binding assay[3]
• for end labeling of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) for electrophoretic motility shift assay[4] and labelling of ATP citrate lyase gene for gel shift assay[5]

for end labeling of DNA for fragmentation reactions[6]

Quality

Typical analysis: 85% DIG-11-ddUTP (HPLC, area%)
Function tetsted by end labeling.

Formula: C₄₃H₆₁N₄O₂₀P3Li₄

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: does not flash
• flash_point_c: does not flash