MilliporeSigma
All Photos(1)

11371517001

Roche

Swa I

from Staphylococcus warneri

Quality Level

form

solution

packaging

pkg of 200 U (10 U/μl)

manufacturer/tradename

Roche

parameter

25 °C optimum reaction temp.

shipped in

dry ice

storage temp.

−20°C

Specificity

Recognition sites: ATTTAAAT
ATTTAAAT
Restriction site: ATTT↓AAAT
ATTT↓AAAT
Heat inactivation: Swa I can be heat inactivated by incubation at 65 °C for 20 minutes.

Application

Swa I is very useful for digesting GC-rich bacterial genomes.

DNA Profile

Number of cleavage sites on different DNAs
  • λ: 0
  • φX174: 0
  • Ad2: 1
  • M13mp7: 1
  • pBR322: 0
  • pBR328: 0
  • SV40: 1

Unit Definition

One unit is the enzyme activity that completely cleaves 1 μg Ad2 × Ssp I DNA in one hour at +25 °C in a total volume of 25 μl (1x) SuRE/Cut buffer H.

Analysis Note

SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity
  • A: 0-10%
  • B: 10-25%
  • H: 100%
  • L: 0-10%
  • M: 0-10%

Activity in PCR buffer: Not tested

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Enzyme Solution

  • SuRE/Cut Buffer H 10x concentrated

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service